Exploration of probiotics on antioxidant activity of serum NLC coenzyme Q10 using the DPPH (2,2diphenyl-1-picrylhidrazyl) method

Background: The skin ageing process can be slowed by routinely caring for the skin using serum cosmetics containing antioxidants such as Co-enzyme Q10 (CoQ10). However, CoQ10 has a drawback, which is low penetration into the skin. In this study, an antiaging serum preparation with the active ingredient NLC-CoQ10 was prepared. Probiotics were added to this preparation as a second active ingredient to enhance antioxidant activity. Objective: This study aimed to analyse the effect of probiotic concentration on the physical characteristics and antioxidant activity of NLC-CoQ10 serum. Method: NLCCoQ10 was mixed with serum base and 0% probiotic (Formula One), 2% probiotic (Formula Two), and 4% probiotic (Formula Three). After that, physical characteristics and antioxidant activity were observed. Results: The results show that the physical characteristics of all formulas fulfil the specifications. All formulas had pH values between 4 and 7 and a viscosity value between 230 and 1150 cPs. The formula with the highest viscosity value is F1, 352.00 +/- 2.00. All formulas have statistically different IC50 50 values. IC50 values for F1, F2 and F3 respectively, are 317.809 +/- 2.117; 307.985 +/- 2.089; 257.549 +/- 1.573. . Conclusion: The antioxidant activity increases with increasing concentrations of probiotics in NLC-CoQ1 0 serum. Meanwhile, the viscosity value decreases with a decreasing concentration of probiotics.