Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience

被引：0

作者：

Cummings, Christopher B. ^{[1
,2
]}

Catania, Samuel S. ^{[1
,2
]}

Ednacot, Eirene M. Q. ^{[1
,2
]}

Kinsella-Johnson, Austin J. ^{[1
,2
]}

Meeds, Claire E. ^{[1
,2
]}

Reynolds, Jack W. ^{[1
,2
]}

Sanderson, Ava E. ^{[1
,2
]}

Johnson, Rachel A. ^{[1
,2
]}

Watts, Katharine R. ^{[1
,2
]}

机构：

[1] Calif Polytech State Univ San Luis Obispo, Dept Chem & Biochem, San Luis Obispo, CA 43407 USA

[2] Calif Polytech State Univ San Luis Obispo, Ctr Applicat Biotechnol, San Luis Obispo, CA 93407 USA

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2024年 / 213期

基金：

美国国家科学基金会;

关键词：

BIOLOGY;

D O I：

10.3791/67067

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The continuous advancement of molecular biology techniques requires that molecular biology curricula are regularly refined to effectively prepare students to enter the workforce with modern competencies. In particular, the emergence of Gibson Assembly, a highly customizable and adaptive molecular cloning technique, has advanced the landscape of molecular cloning in numerous research environments. Thus, we created a Gibson Assembly cloning module for deployment in a molecular and evaluated student learning outcomes from the module. Over three iterations of the course, students participated in an experiment-based independent project that involved cloning three unique plasmid libraries to support research projects in natural products biosynthesis. Students were given pre- and post-questionnaires to evaluate their understanding of molecular cloning and their confidence in molecular biology terms and techniques. Students' responses showed a significant increase in both learning molecular cloning concepts and in self-reported confidence with molecular cloning terms and techniques. This module framework can be generalized to teach Gibson Assembly for various applications, providing instructors with a toolkit for teaching an adaptable and emergent cloning technology while advancing their research projects.

引用

页数：21