Optimization of phenolic compounds extraction and antioxidant activity from Moroccan Crocus sativus L. by-products using predictive modeling and Box-Behnken design

被引：0

作者：

Slimani, Chaimae ^{[1
,2
,3
]}

Fadil, Mouhcine ^{[4
]}

Rais, Chaimae ^{[3
,5
]}

Ullah, Riaz ^{[6
]}

Iqbal, Zafar ^{[7
]}

Santanatoglia, Agnese ^{[8
]}

Caprioli, Giovanni ^{[8
]}

Benjelloun, Meryem ^{[2
]}

Lazraq, Abderrahim ^{[2
]}

Bouyahya, Abdelhakim ^{[9
]}

机构：

[1] Abdelmalek Essaadi Univ, Fac Sci & Tech Al Hoceima, Dept Biol, Lab Res & Dev Engn Sci,Biotechnol Environm Technol, Tetouan, Morocco

[2] Sidi Mohamed Ben Abdellah Univ, Fac Sci & Technol, Dept Biol, Lab Funct Ecol & Environm Engn, Box 2202 Route Imouzzer, Fes, Morocco

[3] Natl Agcy Med & Aromat Plants, Lab Bot, POB 159, Taounate 10, Morocco

[4] Sidi Mohamed Ben Abdellah Univ, Fac Sci & Technol, Dept Biol, Lab Appl Organ Chem, POB 2202,Route Imouzzer, Fes, Morocco

[5] Euromed Univ Fez, UEMF, Fes, Morocco

[6] King Saud Univ, Coll Pharm, Dept Pharmacognosy, Riyadh, Saudi Arabia

[7] King Saud Univ, Coll Med, Dept Surg, POB 7805, Riyadh 11472, Saudi Arabia

[8] Univ Camerino, Sch Pharm, Chem Interdisciplinary Project ChIP, Via Madonna Carceri 9 B, I-62032 Camerino, MC, Italy

[9] Mohammed V Univ Rabat, Fac Sci, Dept Biol, Lab Human Pathol Biol, Rabat, Morocco

来源：

MICROCHEMICAL JOURNAL | 2025年 / 212卷

关键词：

Crocus sativus L; Saffron by-products; Phenolic compounds; Antioxidant activity; Box-Behnken design; Response Surface Methodology (RSM); PRESSURIZED LIQUID EXTRACTION; POLYSACCHARIDES; TEMPERATURE; PARAMETERS; SOLVENT;

D O I：

10.1016/j.microc.2025.113444

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The health benefits of natural antioxidants, particularly phenolic compounds, present in Crocus sativus L. (saffron) are well documented. However, optimizing their extraction from saffron by-products poses significant challenges. This study aimed, for the first time, to enhance the extraction efficiency of phenolic compounds from Moroccan saffron by-products using ultrasound-assisted extraction and Response Surface Methodology (RSM). The Box-Behnken design was employed to optimize three independent variables: solvent-to-material ratio, temperature and extraction time. The antioxidant activity of the extracts was determined using DPPH radical scavenging capacity and the total phenolic content was measured via the Folin-Ciocalteu assay. Optimal extraction conditions were identified as a solvent-to-material ratio of 40 mg/30 mL, with a temperature of 50 degrees C and an extraction duration of 15 min, resulting in extracts with enhanced phenolic content and antioxidant activity. This study highlights the potential of saffron by-products as a valuable source of bioactive compounds and emphasizes the utility of RSM in optimizing extraction processes.

引用

页数：10

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