Identification of CircRNA Complexes Acting on miRNA Sponges within the Exosome in Patients with Alzheimer's Disease: An In Silico Approach

Objective: Alzheimer's disease (AD) is a neurodegenerative ailment that launches insidiously and progresses slowly, and its prevalence gradually increases with age. In a former study, we explored the plasma exosomal circular RNA (circRNA)-micro RNAs (miRNA) expression profiles in AD patients derived from next-generation sequencing data by scanning experimentally validated miRNA-target interaction databases. The current paper focused on computing the integration of circRNA-miRNA and RNA binding proteins (RBP) into the plasma exosomes using the output of our earlier study. Methods: We identified 24 upregulated circRNAs and 12 downregulated miRNAs from our previous paper. These were then subjected to prediction of circRNA-miRNA via the circMine web server and MiRNet. Subsequently, the circRNA Interactome web server was used to integrate the 24 circRNAs with RBPs. Finally, the obtained numeric scale results were visualized using R studio, ensuring the validity and reliability of our findings. Results: As an overall outcome of calculations, we found CNTN4, SH3BGRL, THOC2, CGGBP1, FLNA, ATP6V1A, and UBN1 from queried 24 circRNAs linked to AD pathology, proposing that circRNAs complexes composed of RBPs and miRNAs might be considered empirically under oncoming studies.