Conservation of Putative Liquid-Liquid Phase Separating Proteins in Multiple Drug-Resistant Mycobacterium tuberculosis: Role in Host-Pathogen Interactions?

被引：0

作者：

Singh, Jasdeep ^{[1
,7
]}

Pradhan, Prashant ^{[2
]}

Kataria, Arti ^{[3
]}

Sinha, Sanjeev ^{[4
]}

Ehtesham, Nasreen Z. ^{[5
]}

Monk, Peter N. ^{[6
]}

Hasnain, Seyed E. ^{[5
,7
]}

机构：

[1] Univ Denver, Dept Chem & Biochem, Denver, CO 80210 USA

[2] Univ Texas Southwestern Med Ctr, Cecil H & Ida Green Ctr Reprod Biol Sci, Hamon Ctr Regenerat Sci & Med,Dept Mol Biol,Div Ba, Dept Obstet & Gynecol,Lab Nucl Org,Dept Obstet & G, Dallas, TX 75390 USA

[3] Natl Inst Allergy & Infect Dis NIAID, Natl Inst Hlth NIH, Lab Bacteriol, Rocky Mt Labs, Hamilton, MT 59840 USA

[4] All India Inst Med Sci, Dept Med, Ansari Nagar, New Delhi 110029, India

[5] Sharda Univ, Sch Basic Sci & Res, Dept Life Sci, Greater Noida 201310, Uttar Pradesh, India

[6] Univ Sheffield, Dept Infect Immun & Cardiovasc Dis, Sheffield S10 2TN, England

[7] Indian Inst Technol Delhi, Dept Biochem Engn & Biotechnol, New Delhi 110016, India

来源：

ACS INFECTIOUS DISEASES | 2025年

关键词：

M; tuberculosis; Biomolecular condensates; Liquid-liquid phase separation; Host-pathogeninteractions; Functional genomics; MEMBRANE CHANNEL PROTEIN; PE/PPE MULTIGENE FAMILY;

D O I：

10.1021/acsinfecdis.4c00722

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

We observed a high proportion of proteins in pathogenic Mycobacterium species that can potentially undergo liquid-liquid phase separation (LLPS) mediated biomolecular condensate formation, compared to nonpathogenic species. These proteins mainly include the PE-PPE and PE-PGRS families of proteins that have nucleic acid and protein-protein binding functions, typical of LLPS proteins. We also mapped identified LLPS proteins in M. tuberculosis (M.tb) drug-resistant databases PubMLST and TBProfiler, based upon the WHO 2023 catalogue of resistance-associated mutations. High sequence conservation of LLPS-associated proteins in various multiple drug-resistant M.tb isolates points to their potentially important role in virulence and host-pathogen interactions during pathogenic evolution. This analysis provides a perspective on the role of protein phase separation in the evaluation of M.tb pathogenesis and offers avenues for future research aimed at developing innovative strategies to combat M.tb infection.

引用

页数：8

共 1 条

[1] Drug-resistant strains of Mycobacterium tuberculosis: cell envelope profiles and interactions with the host
Schami, Alyssa
Islam, M. Nurul
Belisle, John T.
Torrelles, Jordi B.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2023, 13

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