共 51 条
Unlocking the Potential of 3D Spheroid Cultures in Breast Cancer Stem Cell Enrichment and Isolation
被引:0
作者:

Ishtiah, Anan A.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Sains Malaysia, Adv Med & Dent Inst IPPT, Dept Biomed Sci, Kepala Batas 13200, Penang, Malaysia Univ Sains Malaysia, Adv Med & Dent Inst IPPT, Dept Biomed Sci, Kepala Batas 13200, Penang, Malaysia

Hairuddin, Omar Nafiis
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Sains Malaysia, Adv Med & Dent Inst IPPT, Dept Biomed Sci, Kepala Batas 13200, Penang, Malaysia Univ Sains Malaysia, Adv Med & Dent Inst IPPT, Dept Biomed Sci, Kepala Batas 13200, Penang, Malaysia

Yahaya, Badrul Hisham
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Sains Malaysia, Adv Med & Dent Inst IPPT, Dept Biomed Sci, Kepala Batas 13200, Penang, Malaysia
Univ Sains Malaysia, Adv Med & Dent Inst IPPT, Breast Canc Translat Res Program, Kepala Batas, Penang, Malaysia Univ Sains Malaysia, Adv Med & Dent Inst IPPT, Dept Biomed Sci, Kepala Batas 13200, Penang, Malaysia
机构:
[1] Univ Sains Malaysia, Adv Med & Dent Inst IPPT, Dept Biomed Sci, Kepala Batas 13200, Penang, Malaysia
[2] Univ Sains Malaysia, Adv Med & Dent Inst IPPT, Breast Canc Translat Res Program, Kepala Batas, Penang, Malaysia
来源:
EURASIAN JOURNAL OF MEDICINE AND ONCOLOGY
|
2024年
/
8卷
/
03期
关键词:
Breast cancer;
cancer resistance;
cancer stem cells;
doxorubicin;
3D culture;
IDENTIFICATION;
CHEMOTHERAPY;
RESISTANCE;
EXPRESSION;
MATRIGEL;
DISEASE;
D O I:
10.14744/ejmo.2024.97120
中图分类号:
R73 [肿瘤学];
学科分类号:
100214 ;
摘要:
Objectives: The present research conducted a comparative analysis of the efficacy of three-dimensional (3D) culturing methods in enriching and isolating breast cancer stem cells (BCSCs). The study compared multicellular spheroids grown in Matrigel and in suspension with the commonly used two-dimensional (2D) monolayer culturing method. Methods: The experiment involved a 9-day 3D multicellular spheroid culture, followed by a 24-hour monolayer culture using two breast cancer cell lines, namely MCF7 and MDA-MB-231. To evaluate BCSCs, the study assessed the expression of various surface markers, including CD44/CD24, Vimentin, and ALDH1, along with pluripotent stem cell genes like SOX2, OCT4, KLF4, and Nanog. Additionally, the Doxorubicin resistance and the capacity of single cells derived from each method to form spheroids in serum-free suspension culture were measured. Results: The findings revealed that 3D-cultured multicellular spheroids grown in suspension exhibited a significant increase in stem cell markers and Doxorubicin resistance. Furthermore, these spheroids demonstrated a higher ability to form single-cell spheroids with a size of more than 50 mu m in a serum-free medium. Conclusion: Overall, this method of 3D culturing in suspension resulted in a substantial enrichment of BCSCs with enhanced self-renewal and proliferation capabilities when compared to both the 2D monolayer and 3D Matrigel methods. Consequently, this approach can serve as a crucial preliminary step in isolating BCSCs from cell lines using any available BCSCs isolation method.
引用
收藏
页码:322 / 335
页数:14
相关论文
共 51 条
[1]
Devices and techniques used to obtain and analyze three-dimensional cell cultures
[J].
Agrawal, Gatika
;
Ramesh, Anuradha
;
Aishwarya, Pargaonkar
;
Sally, Jennifer
;
Ravi, Maddaly
.
BIOTECHNOLOGY PROGRESS,
2021, 37 (03)

Agrawal, Gatika
论文数: 0 引用数: 0
h-index: 0
机构:
Sri Ramachandra Inst Higher Educ & Res, Dept Human Genet, Fac Biomed Sci, Chennai 600116, Tamil Nadu, India Sri Ramachandra Inst Higher Educ & Res, Dept Human Genet, Fac Biomed Sci, Chennai 600116, Tamil Nadu, India

Ramesh, Anuradha
论文数: 0 引用数: 0
h-index: 0
机构:
Sri Ramachandra Inst Higher Educ & Res, Dept Human Genet, Fac Biomed Sci, Chennai 600116, Tamil Nadu, India Sri Ramachandra Inst Higher Educ & Res, Dept Human Genet, Fac Biomed Sci, Chennai 600116, Tamil Nadu, India

Aishwarya, Pargaonkar
论文数: 0 引用数: 0
h-index: 0
机构:
Sri Ramachandra Inst Higher Educ & Res, Dept Human Genet, Fac Biomed Sci, Chennai 600116, Tamil Nadu, India Sri Ramachandra Inst Higher Educ & Res, Dept Human Genet, Fac Biomed Sci, Chennai 600116, Tamil Nadu, India

Sally, Jennifer
论文数: 0 引用数: 0
h-index: 0
机构:
Sri Ramachandra Inst Higher Educ & Res, Dept Human Genet, Fac Biomed Sci, Chennai 600116, Tamil Nadu, India Sri Ramachandra Inst Higher Educ & Res, Dept Human Genet, Fac Biomed Sci, Chennai 600116, Tamil Nadu, India

Ravi, Maddaly
论文数: 0 引用数: 0
h-index: 0
机构:
Sri Ramachandra Inst Higher Educ & Res, Dept Human Genet, Fac Biomed Sci, Chennai 600116, Tamil Nadu, India Sri Ramachandra Inst Higher Educ & Res, Dept Human Genet, Fac Biomed Sci, Chennai 600116, Tamil Nadu, India
[2]
Prospective identification of tumorigenic breast cancer cells
[J].
Al-Hajj, M
;
Wicha, MS
;
Benito-Hernandez, A
;
Morrison, SJ
;
Clarke, MF
.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
2003, 100 (07)
:3983-3988

Al-Hajj, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Michigan, Sch Med, Dept Internal Med, Ctr Comprehens Canc, Ann Arbor, MI 48109 USA

Wicha, MS
论文数: 0 引用数: 0
h-index: 0
机构: Univ Michigan, Sch Med, Dept Internal Med, Ctr Comprehens Canc, Ann Arbor, MI 48109 USA

Benito-Hernandez, A
论文数: 0 引用数: 0
h-index: 0
机构: Univ Michigan, Sch Med, Dept Internal Med, Ctr Comprehens Canc, Ann Arbor, MI 48109 USA

Morrison, SJ
论文数: 0 引用数: 0
h-index: 0
机构: Univ Michigan, Sch Med, Dept Internal Med, Ctr Comprehens Canc, Ann Arbor, MI 48109 USA

Clarke, MF
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Michigan, Sch Med, Dept Internal Med, Ctr Comprehens Canc, Ann Arbor, MI 48109 USA Univ Michigan, Sch Med, Dept Internal Med, Ctr Comprehens Canc, Ann Arbor, MI 48109 USA
[3]
Alterations in CD44 isoforms and HAS expression in human articular chondrocytes during the de- and re-differentiation processes
[J].
Albrecht, Christian
;
Schlegel, Werner
;
Eckl, Peter
;
Jagersberger, Tamara
;
Sadeghi, Kambis
;
Berger, Angelika
;
Vecsei, Vilmos
;
Marlovits, Stefan
.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,
2009, 23 (02)
:253-259

Albrecht, Christian
论文数: 0 引用数: 0
h-index: 0
机构:
Salzburg Univ, Dept Cell Biol, A-5020 Salzburg, Austria
Med Univ Vienna, Dept Traumatol, Ctr Joint & Cartilage, Austrian Cluster Tissue Regenerat, A-1090 Vienna, Austria Salzburg Univ, Dept Cell Biol, A-5020 Salzburg, Austria

Schlegel, Werner
论文数: 0 引用数: 0
h-index: 0
机构:
Med Univ Vienna, Dept Paediat, A-1090 Vienna, Austria
Med Univ Vienna, Dept Traumatol, Ctr Joint & Cartilage, Austrian Cluster Tissue Regenerat, A-1090 Vienna, Austria Salzburg Univ, Dept Cell Biol, A-5020 Salzburg, Austria

Eckl, Peter
论文数: 0 引用数: 0
h-index: 0
机构:
Salzburg Univ, Dept Cell Biol, A-5020 Salzburg, Austria Salzburg Univ, Dept Cell Biol, A-5020 Salzburg, Austria

Jagersberger, Tamara
论文数: 0 引用数: 0
h-index: 0
机构:
Med Univ Vienna, Dept Traumatol, Ctr Joint & Cartilage, Austrian Cluster Tissue Regenerat, A-1090 Vienna, Austria Salzburg Univ, Dept Cell Biol, A-5020 Salzburg, Austria

Sadeghi, Kambis
论文数: 0 引用数: 0
h-index: 0
机构:
Med Univ Vienna, Dept Paediat, A-1090 Vienna, Austria Salzburg Univ, Dept Cell Biol, A-5020 Salzburg, Austria

Berger, Angelika
论文数: 0 引用数: 0
h-index: 0
机构:
Med Univ Vienna, Dept Traumatol, Ctr Joint & Cartilage, Austrian Cluster Tissue Regenerat, A-1090 Vienna, Austria Salzburg Univ, Dept Cell Biol, A-5020 Salzburg, Austria

Vecsei, Vilmos
论文数: 0 引用数: 0
h-index: 0
机构:
Med Univ Vienna, Dept Traumatol, Ctr Joint & Cartilage, Austrian Cluster Tissue Regenerat, A-1090 Vienna, Austria Salzburg Univ, Dept Cell Biol, A-5020 Salzburg, Austria

Marlovits, Stefan
论文数: 0 引用数: 0
h-index: 0
机构:
Med Univ Vienna, Dept Traumatol, Ctr Joint & Cartilage, Austrian Cluster Tissue Regenerat, A-1090 Vienna, Austria Salzburg Univ, Dept Cell Biol, A-5020 Salzburg, Austria
[4]
Cancer Stem Cells and Targeting Strategies
[J].
Barbato, Luisa
;
Bocchetti, Marco
;
Di Biase, Anna
;
Regad, Tarik
.
CELLS,
2019, 8 (08)

Barbato, Luisa
论文数: 0 引用数: 0
h-index: 0
机构:
Nottingham Trent Univ, John van Geest Canc Res Ctr, Sch Sci & Technol, Clifton Lane, Nottingham NG11 8NS, England Nottingham Trent Univ, John van Geest Canc Res Ctr, Sch Sci & Technol, Clifton Lane, Nottingham NG11 8NS, England

Bocchetti, Marco
论文数: 0 引用数: 0
h-index: 0
机构:
Nottingham Trent Univ, John van Geest Canc Res Ctr, Sch Sci & Technol, Clifton Lane, Nottingham NG11 8NS, England
Univ Campania Luigi Vanvitelli, Dept Precis Med, I-80138 Naples, Italy Nottingham Trent Univ, John van Geest Canc Res Ctr, Sch Sci & Technol, Clifton Lane, Nottingham NG11 8NS, England

论文数: 引用数:
h-index:
机构:

论文数: 引用数:
h-index:
机构:
[5]
Matrigel: From discovery and ECM mimicry to assays and models for cancer research
[J].
Benton, Gabriel
;
Arnaoutova, Irina
;
George, Jay
;
Kleinman, Hynda K.
;
Koblinski, Jennifer
.
ADVANCED DRUG DELIVERY REVIEWS,
2014, 79-80
:3-18

Benton, Gabriel
论文数: 0 引用数: 0
h-index: 0
机构:
Trevigen Inc, 8405 Helgerman Ct, Gaithersburg, MD 20877 USA Trevigen Inc, 8405 Helgerman Ct, Gaithersburg, MD 20877 USA

Arnaoutova, Irina
论文数: 0 引用数: 0
h-index: 0
机构:
Trevigen Inc, 8405 Helgerman Ct, Gaithersburg, MD 20877 USA Trevigen Inc, 8405 Helgerman Ct, Gaithersburg, MD 20877 USA

George, Jay
论文数: 0 引用数: 0
h-index: 0
机构:
Trevigen Inc, 8405 Helgerman Ct, Gaithersburg, MD 20877 USA Trevigen Inc, 8405 Helgerman Ct, Gaithersburg, MD 20877 USA

Kleinman, Hynda K.
论文数: 0 引用数: 0
h-index: 0
机构:
George Washington Univ, Sch Med, Washington, DC 20037 USA Trevigen Inc, 8405 Helgerman Ct, Gaithersburg, MD 20877 USA

Koblinski, Jennifer
论文数: 0 引用数: 0
h-index: 0
机构:
Virginia Commonwealth Univ, Richmond, VA 23220 USA Trevigen Inc, 8405 Helgerman Ct, Gaithersburg, MD 20877 USA
[6]
Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell
[J].
Bonnet, D
;
Dick, JE
.
NATURE MEDICINE,
1997, 3 (07)
:730-737

Bonnet, D
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV TORONTO, DEPT MOL & MED GENET, TORONTO, ON M5G 1X8, CANADA UNIV TORONTO, DEPT MOL & MED GENET, TORONTO, ON M5G 1X8, CANADA

Dick, JE
论文数: 0 引用数: 0
h-index: 0
机构:
UNIV TORONTO, DEPT MOL & MED GENET, TORONTO, ON M5G 1X8, CANADA UNIV TORONTO, DEPT MOL & MED GENET, TORONTO, ON M5G 1X8, CANADA
[7]
High-throughput screening in multicellular spheroids for target discovery in the tumor microenvironment
[J].
Calpe, Blaise
;
Kovacs, Werner J.
.
EXPERT OPINION ON DRUG DISCOVERY,
2020, 15 (08)
:955-967

Calpe, Blaise
论文数: 0 引用数: 0
h-index: 0
机构:
Swiss Fed Inst Technol, Inst Mol Hlth Sci, Otto Stern Weg 7,HPL H16-1, CH-8093 Zurich, Switzerland
Debiopharm, Dept Biol, Lausanne, Switzerland Swiss Fed Inst Technol, Inst Mol Hlth Sci, Otto Stern Weg 7,HPL H16-1, CH-8093 Zurich, Switzerland

Kovacs, Werner J.
论文数: 0 引用数: 0
h-index: 0
机构:
Swiss Fed Inst Technol, Inst Mol Hlth Sci, Otto Stern Weg 7,HPL H16-1, CH-8093 Zurich, Switzerland Swiss Fed Inst Technol, Inst Mol Hlth Sci, Otto Stern Weg 7,HPL H16-1, CH-8093 Zurich, Switzerland
[8]
In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel
[J].
Casagrande, Naike
;
Borghese, Cinzia
;
Agostini, Francesco
;
Durante, Cristina
;
Mazzucato, Mario
;
Colombatti, Alfonso
;
Aldinucci, Donatella
.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,
2021, 22 (06)
:1-11

Casagrande, Naike
论文数: 0 引用数: 0
h-index: 0
机构:
IRCCS, Ctr Riferimento Oncol Aviano CRO, Mol Oncol Unit, I-33081 Aviano, Italy IRCCS, Ctr Riferimento Oncol Aviano CRO, Mol Oncol Unit, I-33081 Aviano, Italy

Borghese, Cinzia
论文数: 0 引用数: 0
h-index: 0
机构:
IRCCS, Ctr Riferimento Oncol Aviano CRO, Mol Oncol Unit, I-33081 Aviano, Italy IRCCS, Ctr Riferimento Oncol Aviano CRO, Mol Oncol Unit, I-33081 Aviano, Italy

Agostini, Francesco
论文数: 0 引用数: 0
h-index: 0
机构:
IRCCS, Ctr Riferimento Oncol Aviano CRO, Stem Cell Unit, I-33081 Aviano, Italy IRCCS, Ctr Riferimento Oncol Aviano CRO, Mol Oncol Unit, I-33081 Aviano, Italy

Durante, Cristina
论文数: 0 引用数: 0
h-index: 0
机构:
IRCCS, Ctr Riferimento Oncol Aviano CRO, Stem Cell Unit, I-33081 Aviano, Italy IRCCS, Ctr Riferimento Oncol Aviano CRO, Mol Oncol Unit, I-33081 Aviano, Italy

Mazzucato, Mario
论文数: 0 引用数: 0
h-index: 0
机构:
IRCCS, Ctr Riferimento Oncol Aviano CRO, Stem Cell Unit, I-33081 Aviano, Italy IRCCS, Ctr Riferimento Oncol Aviano CRO, Mol Oncol Unit, I-33081 Aviano, Italy

Colombatti, Alfonso
论文数: 0 引用数: 0
h-index: 0
机构:
IRCCS, Ctr Riferimento Oncol Aviano CRO, Mol Oncol Unit, I-33081 Aviano, Italy IRCCS, Ctr Riferimento Oncol Aviano CRO, Mol Oncol Unit, I-33081 Aviano, Italy

Aldinucci, Donatella
论文数: 0 引用数: 0
h-index: 0
机构:
IRCCS, Ctr Riferimento Oncol Aviano CRO, Mol Oncol Unit, I-33081 Aviano, Italy IRCCS, Ctr Riferimento Oncol Aviano CRO, Mol Oncol Unit, I-33081 Aviano, Italy
[9]
Proteomic analysis of the proteins that are associated with the resistance to paclitaxel in human breast cancer cells
[J].
Chen, Siying
;
Dong, Qian
;
Hu, Sasa
;
Cai, Jiangxia
;
Zhang, Weipeng
;
Sun, Jinyao
;
Wang, Taotao
;
Xie, Jiao
;
He, Hairong
;
Xing, Jianfeng
;
Lu, Jun
;
Dong, Yalin
.
MOLECULAR BIOSYSTEMS,
2014, 10 (02)
:294-303

Chen, Siying
论文数: 0 引用数: 0
h-index: 0
机构:
Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China

Dong, Qian
论文数: 0 引用数: 0
h-index: 0
机构:
Qilu Pharmaceut Co Ltd, Jinan, Shandong, Peoples R China Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China

Hu, Sasa
论文数: 0 引用数: 0
h-index: 0
机构:
Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China

Cai, Jiangxia
论文数: 0 引用数: 0
h-index: 0
机构:
Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China

Zhang, Weipeng
论文数: 0 引用数: 0
h-index: 0
机构:
Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China

Sun, Jinyao
论文数: 0 引用数: 0
h-index: 0
机构:
Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China

Wang, Taotao
论文数: 0 引用数: 0
h-index: 0
机构:
Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China

Xie, Jiao
论文数: 0 引用数: 0
h-index: 0
机构:
Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China

He, Hairong
论文数: 0 引用数: 0
h-index: 0
机构:
Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China

Xing, Jianfeng
论文数: 0 引用数: 0
h-index: 0
机构:
Xi An Jiao Tong Univ, Coll Med, Dept Pharm, Xian 710061, Shaanxi, Peoples R China Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China

Lu, Jun
论文数: 0 引用数: 0
h-index: 0
机构:
Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China

Dong, Yalin
论文数: 0 引用数: 0
h-index: 0
机构:
Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China Xi An Jiao Tong Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Xian 710061, Shaanxi, Peoples R China
[10]
Breast Cancer Stem Cell Membrane Biomarkers: Therapy Targeting and Clinical Implications
[J].
Conde, Ines
;
Ribeiro, Ana Sofia
;
Paredes, Joana
.
CELLS,
2022, 11 (06)

Conde, Ines
论文数: 0 引用数: 0
h-index: 0
机构:
Inst Invest & Innovat Hlth, I3S, P-4200135 Porto, Portugal
Univ Porto, Inst Mol Pathol & Immunol, Ipatimup, P-4200135 Porto, Portugal Inst Invest & Innovat Hlth, I3S, P-4200135 Porto, Portugal

Ribeiro, Ana Sofia
论文数: 0 引用数: 0
h-index: 0
机构:
Inst Invest & Innovat Hlth, I3S, P-4200135 Porto, Portugal
Univ Porto, Inst Mol Pathol & Immunol, Ipatimup, P-4200135 Porto, Portugal Inst Invest & Innovat Hlth, I3S, P-4200135 Porto, Portugal

Paredes, Joana
论文数: 0 引用数: 0
h-index: 0
机构:
Inst Invest & Innovat Hlth, I3S, P-4200135 Porto, Portugal
Univ Porto, Inst Mol Pathol & Immunol, Ipatimup, P-4200135 Porto, Portugal
Univ Porto, Fac Med, P-4200319 Porto, Portugal Inst Invest & Innovat Hlth, I3S, P-4200135 Porto, Portugal