Effects of high-intensity interval training and moderate-intensity continuous training on neural dynamics and firing in the CA1-MEC region of mice

The aim of this study is to investigate the differential impacts of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on neural circuit dynamics and neuronal firing in the hippocampal CA1 subregion (CA1) region and medial entorhinal cortex (MEC) of mice. Forty-two male ICR mice were randomized into control, HIIT, and MICT groups. Electrophysiological recordings were performed pre- and postintervention to assess neural circuit dynamics and neuronal firing patterns in the CA1-MEC pathway. Both exercise protocols increased local field potential (LFP) coherence, with MICT showing a more pronounced effect on delta and gamma coherences (P < 0.05). Both modalities reduced delta power spectral density (PSD) (HIIT, P < 0.05; MICT, P < 0.01) and elevated theta, beta, and gamma PSDs. Neuronal firing frequency improved in both CA1 and MEC following HIIT and MICT (P < 0.05). HIIT enhanced firing regularity in CA1 (P < 0.05), whereas MICT improved regularity in both regions (P < 0.05). Both protocols reduced firing latency (HIIT, P < 0.05; MICT, P < 0.01) and enhanced burst firing ratio, interburst interval (IBI), burst duration (BD), and LFP phase locking (P < 0.05 or P < 0.01). Notably, MICT significantly improved spatial working memory and novel recognition abilities, as evidenced by increased novel arm time, entries, and preference index (P < 0.01). This study reveals that both HIIT and MICT positively impact neural processing and information integration in the CA1-MEC network of mice. Notably, MICT exhibits a more pronounced impact on neural functional connectivity and cognitive function compared with HIIT. These findings, coupled with the similarities in hippocampal electrophysiological characteristics between rodents and humans, suggest potential exercise-mediated neural plasticity and cognitive benefits in humans.