Temperature-controlled electrospray ionization tandem mass spectrometry study on protein/small molecule interaction

被引：0
作者：
Su, Wen [1 ,2 ]
Liu, Siying [1 ]
Zhang, Qingfu [1 ]
Zhou, Zhongyan [1 ]
Wang, Na [1 ,3 ]
Yue, Lei [1 ]
机构：
[1] Hunan Univ, Coll Biol, Dept Pharm, State Key Lab Chemo Biosensing & Chemometr, Changsha 410000, Peoples R China
[2] Shaoyang Univ, Coll Pharm, Shaoyang 422000, Peoples R China
[3] Hunan Univ Sci & Technol, Sch Chem & Chem Engn, Xiangtan 411100, Peoples R China
来源：
CHINESE CHEMICAL LETTERS | 2025年 / 36卷 / 05期
基金：
中国国家自然科学基金;
关键词：
Protein-small molecule interaction; Electrospray ionization; Tandem mass spectrometry; Ubiquitin; Flavonol; BINDING; FLAVONOIDS; COMPLEXES; UBIQUITIN; DNA; STOICHIOMETRY; DENATURATION; ASSOCIATIONS; ASTRINGENCY; STABILITY;
D O I：
10.1016/j.cclet.2024.110237
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Traditional electrospray ionization tandem mass spectrometry (ESI-MSn) has been a powerful tool in diverse research areas, however, it faces great limitations in the study of protein-small molecule interactions. In this article, the state-of-the-art temperature-controlled electrospray ionization tandem mass spectrometry (TC-ESI-MSn) is applied to investigate interactions between ubiquitin and two flavonol molecules, respectively. The combination of collision-induced dissociation (CID) and MS solution-melting experiments facilitates the understanding of flavonol-protein interactions in a new dimension across varying temperature ranges. While structural changes of proteins disturbed by small molecules are unseen in ESI-MSn, TC-ESI-MSn allows a simultaneous assessment of the stability of the complex in both gas and liquid phases under various temperature conditions, meanwhile investigating the impact on the protein's structure and tracking changes in thermodynamic data, and the characteristics of structural intermediates. (c) 2025 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
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页数：5
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共 72 条
  • [1] Chen F., Gulbakan B., Weidmann S., Et al., Mass Spectrom. Rev., 35, pp. 48-70, (2016)
  • [2] Loo J.A., Mass Spectrom. Rev., 16, pp. 1-23, (1997)
  • [3] Bennett J.L., Nguyen G.T., Donald W.A., Chem. Rev., 122, pp. 7327-7385, (2022)
  • [4] Lourenco C.F., Gago B., Barbosa R.M., Et al., J. Agric. Food Chem., 56, pp. 3798-3804, (2008)
  • [5] Peterson J.J., Dwyer J.T., Jacques P.F., Et al., Nutr. Rev., 70, pp. 491-508, (2012)
  • [6] Xin P., Han S., Huang J., Et al., Chin. Chem. Lett., 34, (2023)
  • [7] Treutter D., Environ. Chem. Lett., 4, pp. 147-157, (2006)
  • [8] Laoue J., Fernandez C., Ormeno E., Plants, 11, (2022)
  • [9] Zhang X., Li J., Lu K.Z., Et al., Chin. Chem. Lett., 35, (2024)
  • [10] Kanakis C.D., Hasni I., Bourassa P., Et al., Food Chem., 127, pp. 1046-1055, (2011)
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