The basic leucine-zipper transcription factor DlbZIP36 is involved in regulating flowering in longan

被引：0

作者：

Wang, Jinying ^{[2
]}

Fan, Zhiyi ^{[2
]}

Qing, Haowei ^{[2
]}

Shi, Yuyu ^{[1
]}

Mo, Xiao ^{[1
]}

Guo, Huiqin ^{[1
]}

Yi, Chenxin ^{[1
]}

Wei, Jinju ^{[1
]}

Jia, Haifeng ^{[2
]}

Zhang, Yanqing ^{[2
]}

Qiu, Haiji ^{[2
]}

Xu, Jiongzhi ^{[2
]}

Pan, Jiechun ^{[2
]}

Ding, Feng ^{[1
,2
]}

Zhang, Shuwei ^{[1
]}

机构：

[1] Guangxi Acad Agr Sci, Guangxi Key Lab Genet Improvement Crops, Nanning 530007, Peoples R China

[2] Guangxi Univ, Agr & Anim Husb Ind Dev Res Inst, Coll Agr, State Key Lab Conservat & Utilizat Subtrop Agrobio, Nanning 530004, Peoples R China

来源：

SCIENTIA HORTICULTURAE | 2025年 / 343卷

基金：

中国国家自然科学基金;

关键词：

Longan; bZIP; Flowering; Expression analysis; Ethephon and paclobutrazol; FUNCTIONAL-CHARACTERIZATION; EXPRESSION ANALYSIS; GENE FAMILY; BZIP; PROTEIN; PROMOTER; LEAF; ABA; INFORMATION; RESISTANCE;

D O I：

10.1016/j.scienta.2025.114089

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Longan (Dimocarpus longan Lour.) is a well-known subtropical fruit crop. However, the narrow harvest window seriously affects its economic benefits. Flowering time is a key factor that determines the ripening time of longan. Previous studies have demonstrated that ethephon and paclobutrazol treatment can regulate the flowering time of 'Sijimi' longan, and further transcriptomic analysis found that the basic leucine zipper (bZIP) transcription factor family may play a key role in this regulation process. Here, to reveal the functions of bZIP proteins in 'Sijimi' longan, we cloned 39 DlbZIP genes identified by a genome wide domain analysis, which were designated as DlbZIP1-39. Analysis of the temporal and spatial expression patterns of these genes showed that DlbZIP36 had higher expression in petioles, and the expression was significantly enhanced by ethephon and paclobutrazol treatments. Subcellular localization analysis showed that DlbZIP36 is located in the nucleus. Finally, ectopic overexpression of DlbZIP36 in Arabidopsis resulted in earlier flowering under long day conditions, and led to higher expression of Arabidopsis FLOWERING LOCUS T (AtFT) and FRUITFULL (AtFUL) while lower expression of Arabidopsis FLOWERING LOCUS C (AtFLC) than the wild type. In addition, overexpression of DlbZIP36 in tobacco also resulted in early flowering. These results suggest that DlbZIP36 may enhance the flowering of 'Sijimi' longan under ethephon and paclobutrazol treatment, providing an important theoretical basis for the perinatal regulation and breeding of longan in the future.

引用

页数：15

共 64 条

[1] FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex [J].

Abe, M ;

Kobayashi, Y ;

Yamamoto, S ;

Daimon, Y ;

Yamaguchi, A ;

Ikeda, Y ;

Ichinoki, H ;

Notaguchi, M ;

Goto, K ;

Araki, T .

SCIENCE, 2005, 309 (5737) :1052-1056

[2] A Pivotal Role of the Basic Leucine Zipper Transcription Factor bZIP53 in the Regulation of Arabidopsis Seed Maturation Gene Expression Based on Heterodimerization and Protein Complex Formation [J].

Alonso, Rosario ;

Onate-Sanchez, Luis ;

Weltmeier, Fridtjof ;

Ehlert, Andrea ;

Diaz, Isabel ;

Dietrich, Katrin ;

Vicente-Carbajosa, Jesus ;

Droege-Laser, Wolfgang .

PLANT CELL, 2009, 21 (06) :1747-1761

[3] The genetic basis of flowering responses to seasonal cues [J].

Andres, Fernando ;

Coupland, George .

NATURE REVIEWS GENETICS, 2012, 13 (09) :627-639

[4] Arabidopsis thaliana transcription factors bZIP19 and bZIP23 regulate the adaptation to zinc deficiency [J].

Assuncao, Ana G. L. ;

Herrero, Eva ;

Lin, Ya-Fen ;

Huettel, Bruno ;

Talukdar, Sangita ;

Smaczniak, Cezary ;

Immink, Richard G. H. ;

van Eldik, Mandy ;

Fiers, Mark ;

Schat, Henk ;

Aarts, Mark G. M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (22) :10296-10301

[5] Bioinformatic cis-element analyses performed in Arabidopsis and rice disclose bZIP- and MYB-related binding sites as potential AuxRE-coupling elements in auxin-mediated transcription [J].

Berendzen, Kenneth W. ;

Weiste, Christoph ;

Wanke, Dierk ;

Kilian, Joachim ;

Harter, Klaus ;

Droege-Laser, Wolfgang .

BMC PLANT BIOLOGY, 2012, 12

[6] Flowering time regulation in crops - what did we learn from Arabidopsis? [J].

Bluemel, Martina ;

Dally, Nadine ;

Jung, Christian .

CURRENT OPINION IN BIOTECHNOLOGY, 2015, 32 :121-129

[7] TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data [J].

Chen, Chengjie ;

Chen, Hao ;

Zhang, Yi ;

Thomas, Hannah R. ;

Frank, Margaret H. ;

He, Yehua ;

Xia, Rui .

MOLECULAR PLANT, 2020, 13 (08) :1194-1202

[8] Effects of Adenosine Triphosphate (ATP) Treatment on Postharvest Physiology, Quality and Storage Behavior of Longan Fruit [J].

Chen, Mengyin ;

Lin, Hetong ;

Zhang, Shen ;

Lin, Yifen ;

Chen, Yihui ;

Lin, Yixiong .

FOOD AND BIOPROCESS TECHNOLOGY, 2015, 8 (05) :971-982

[9] Promoter difference of LcFT1 is a leading cause of natural variation of flowering timing in different litchi cultivars (Litchi chinensis Sonn.) [J].

Ding, Feng ;

Zhang, Shuwei ;

Chen, Houbin ;

Su, Zuanxian ;

Zhang, Rong ;

Xiao, Qiusheng ;

Li, Hongli .

PLANT SCIENCE, 2015, 241 :128-137

[10] Isolation and functional characterization of three MiFTs genes from mango [J].

Fan, Zhi-Yi ;

He, Xin-Hua ;

Fan, Yan ;

Yu, Hai-Xia ;

Wang, Yi-Han ;

Xie, Xiao-Jie ;

Liu, Yuan ;

Mo, Xiao ;

Wang, Jin-Ying ;

Luo, Cong .

PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2020, 155 :169-176

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