A novel anti-mouse CCR7 monoclonal antibody, C7Mab-7, demonstrates high sensitivity in flow cytometry, western blot, and immunohistochemistry

被引：0

作者：

Satofuka, Hiroyuki ^{[1
]}

Suzuki, Hiroyuki ^{[1
]}

Tanaka, Tomohiro ^{[1
]}

Ubukata, Rena ^{[1
]}

Hirose, Miu ^{[1
]}

Yamamoto, Haruto ^{[1
]}

Kaneko, Yu ^{[1
]}

Fujisawa, Shiori ^{[1
]}

Li, Guanjie ^{[1
]}

Kaneko, Mika K. ^{[1
]}

Kato, Yukinari ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Med, Dept Antibody Drug Dev, 2-1 Seiryo-Machi,Aoba Ku, Sendai, Miyagi 9808575, Japan

来源：

BIOCHEMISTRY AND BIOPHYSICS REPORTS | 2025年 / 41卷

基金：

日本学术振兴会;

关键词：

Mouse CCR7; Monoclonal antibody; Cell-based immunization and screening; Flow cytometry; Western blot; Immunohistochemistry; CHEMOKINE RECEPTOR CCR7; LYMPH-NODE METASTASIS; EXPRESSION; INFILTRATION; FIBROCYTES; LEUKEMIA; CXCR4; CELLS;

D O I：

10.1016/j.bbrep.2025.101948

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

C-C chemokine receptor type 7 (CCR7) is a member of the G protein-coupled receptor family and functions as a lymph node-homing receptor for immune cells. Upon ligand binding, CCR7 promotes the migration of immune cells to secondary lymphoid organs. In cancers, CCR7 has been revealed as a critical molecule in lymph node metastasis. Consequently, anti-CCR7 monoclonal antibodies (mAbs) have been developed as cancer therapeutic agents. In this study, we established an anti-mouse CCR7 (mCCR7) mAb, C7Mab-7 (rat IgG1, kappa) using the Cell-Based Immunization and Screening (CBIS) method. C7Mab-7 demonstrated high sensitivity in flow cytometry. The dissociation constant (KD) value of C7Mab-7 was determined to be 2.5 x 10-9 M for mCCR7overexpressed Chinese hamster ovary-K1 (CHO/mCCR7) cells. Furthermore, C7Mab-7 detected mCCR7 with high sensitivity in western blot and immunohistochemistry. C7Mab-7, developed by the CBIS method, accelerates the development of CCR7-targeted antibody therapies and cancer diagnostics.

引用

页数：7

共 57 条

[11] Bill C.A., Allen C.M., Vines C.M., C-C chemokine receptor 7 in cancer, Cells, 11, (2022)
[12] Alrumaihi F., The multi-functional roles of CCR7 in human immunology and as a promising therapeutic target for cancer therapeutics, Front. Mol. Biosci., 9, (2022)
[13] Rot A., von Andrian U.H., Chemokines in innate and adaptive host defense: basic chemokinese grammar for immune cells, Annu. Rev. Immunol., 22, pp. 891-928, (2004)
[14] Mburu Y.K., Egloff A.M., Walker W.H., Wang L., Seethala R.R., van Waes C., Ferris R.L., Chemokine receptor 7 (CCR7) gene expression is regulated by NF-kappaB and activator protein 1 (AP1) in metastatic squamous cell carcinoma of head and neck (SCCHN), J. Biol. Chem., 287, pp. 3581-3590, (2012)
[15] Hopken U.E., Foss H.D., Meyer D., Hinz M., Leder K., Stein H., Lipp M., Up-regulation of the chemokine receptor CCR7 in classical but not in lymphocyte-predominant Hodgkin disease correlates with distinct dissemination of neoplastic cells in lymphoid organs, Blood, 99, pp. 1109-1116, (2002)
[16] Ji H., Hu C., Yang X., Liu Y., Ji G., Ge S., Wang X., Wang M., Lymph node metastasis in cancer progression: molecular mechanisms, clinical significance and therapeutic interventions, Signal Transduct. Targeted Ther., 8, (2023)
[17] Muller A., Homey B., Soto H., Ge N., Catron D., Buchanan M.E., McClanahan T., Murphy E., Yuan W., Wagner S.N., Barrera J.L., Mohar A., Verastegui E., Zlotnik A., Involvement of chemokine receptors in breast cancer metastasis, Nature, 410, pp. 50-56, (2001)
[18] Cabioglu N., Yazici M.S., Arun B., Broglio K.R., Hortobagyi G.N., Price J.E., Sahin A., CCR7 and CXCR4 as novel biomarkers predicting axillary lymph node metastasis in T1 breast cancer, Clin. Cancer Res., 11, pp. 5686-5693, (2005)
[19] Shi W., Zou R., Yang M., Mai L., Ren J., Wen J., Liu Z., Lai R., Analysis of genes involved in ulcerative colitis activity and tumorigenesis through systematic mining of gene Co-expression networks, Front. Physiol., 10, (2019)
[20] Irino T., Takeuchi H., Matsuda S., Saikawa Y., Kawakubo H., Wada N., Takahashi T., Nakamura R., Fukuda K., Omori T., Kitagawa Y., CC-Chemokine receptor CCR7: a key molecule for lymph node metastasis in esophageal squamous cell carcinoma, BMC Cancer, 14, (2014)

← 1 2 3 4 5 6 →