Detection of liver mitochondrial oxaloacetate by NMR spectroscopy and membrane potential-dependent accumulation

被引：0
作者：
Yu, Liping [1 ,2 ,3 ,4 ]
Fink, Brian D. [2 ,5 ]
Sivitz, William I. [2 ,5 ]
机构：
[1] Univ Iowa, Dept Mol Physiol & Biophys, Iowa City, IA USA
[2] Iowa City Vet Affairs Med Ctr, Iowa City, IA USA
[3] Univ Iowa, Dept Biochem & Mol Biol, Iowa City, IA USA
[4] Univ Iowa, NMR Core Facil, Iowa City, IA USA
[5] Univ Iowa, Dept Internal Med Endocrinol & Metab, Iowa City, IA USA
来源：
FASEB JOURNAL | 2025年 / 39卷 / 07期
关键词：
inner membrane potential; liver; metabolites; mitochondria; oxaloacetate; respiration;
D O I：
10.1096/fj.202500039R
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Oxaloacetate (OAA) is a central liver metabolite fundamental to critical metabolic pathways. However, understanding OAA metabolism in the liver has been limited because the compound is very difficult to measure by mass spectroscopy and not abundant enough for detection by other methods. Here we describe a novel approach to quantifying OAA in liver mitochondria. Moreover, we provide evidence for membrane potential-dependent OAA accumulation in mitochondria during complex II-energized respiration consistent with OAA inhibition of succinate dehydrogenase.
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页数：5
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