The Gram-negative bacterium Vibrio harveyi is a serious shrimp pathogen. Here, we present the genome sequence of Vibrio harveyi TUMSAT-2019, which was isolated from a kuruma shrimp (Penaeus japonicus) that originated from a shrimp farm in Okinawa Prefecture. The assembly totaled 5.7 Mbp, consisting of two chromosomes with no plasmid.