Diagnostic accuracy comparison of the Fluorion MTBC QLP 2.1 real-time PCR and the COBAS TaqMan MTB assays for Mycobacterium tuberculosis detection

被引：0

作者：

Madihi, Salma ^{[1
]}

Aainouss, Achraf ^{[2
]}

El Messaoudi, Mly Driss ^{[2
]}

Benani, Abdelouaheb ^{[1
]}

机构：

[1] Inst Pasteur Maroc, Mol Biol Lab, Pl Louis Pasteur, Casablanca 20360, Morocco

[2] Inst Pasteur Maroc, Lab Mycobacteria & TB, Casablanca, Morocco

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2025年 / 232卷

关键词：

COBAS TaqMan MTB; Diagnosis; Fluorion MTBC QLP 2.1 real-time PCR; Methods comparison; Mycobacterium tuberculosis;

D O I：

10.1016/j.mimet.2025.107115

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Fluorion MTBC QLP 2.1 Real-Time PCR test is a valuable alternative for tuberculosis diagnosis, demonstrating 100 % specificity and 87.5 % sensitivity for respiratory specimens, 97.8 % specificity and 85.3 sensitivity for non-respiratory specimens, and an overall agreement of 92.3 % with the COBAS TaqMan MTB assay.

引用

页数：3

共 50 条

[31] New Real-Time PCR Assays for Detection of Inducible and Acquired Clarithromycin Resistance in the Mycobacterium abscessus Group [J].

Shallom, Shamira J. ;

Moura, Natalia S. ;

Olivier, Kenneth N. ;

Sampaio, Elizabeth P. ;

Holland, Steven M. ;

Zelazny, Adrian M. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2015, 53 (11) :3430-3437

[32] Comparison of histopathology and real-time polymerase chain reaction (RT-PCR) for detection of Mycobacterium tuberculosis in fistula-in-ano [J].

Pankaj Garg .

International Journal of Colorectal Disease, 2017, 32 :1033-1035

[33] Comparison of histopathology and real-time polymerase chain reaction (RT-PCR) for detection of Mycobacterium tuberculosis in fistula-in-ano [J].

Garg, Pankaj .

INTERNATIONAL JOURNAL OF COLORECTAL DISEASE, 2017, 32 (07) :1033-1035

[34] Rapid Detection of Rifampicin- and Isoniazid-Resistant Mycobacterium tuberculosis Using Real-Time PCR [J].

Sahebi, Leyla ;

Ansarin, Khalil ;

Monfaredan, Amir ;

Farajnia, Safar ;

Nili, Seiran ;

Khalili, Majid .

JUNDISHAPUR JOURNAL OF MICROBIOLOGY, 2016, 9 (10)

[35] Risk factors for Mycobacterium tuberculosis resistance and detection of resistance mutations to Rifampin and Isoniazid by Real-Time PCR [J].

Hristea, Adriana ;

Otelea, Dan ;

Macri, Anca ;

Baicus, Cristian ;

Moldovan, Olga ;

Tinischi, Mihaela ;

Paraschiv, Simona ;

Ruxandra, Moroti ;

Arama, Victoria ;

Streinu-Cercel, Adrian .

REVISTA ROMANA DE MEDICINA DE LABORATOR, 2010, 18 (02) :19-28

[36] Detection of Mycobacterium tuberculosis genotypic groups by a duplex real-time PCR targeting the katG and gyrA genes [J].

Rindi, L ;

Lari, N ;

Bonanni, D ;

Garzelli, C .

JOURNAL OF MICROBIOLOGICAL METHODS, 2004, 59 (02) :283-287

[37] Development and evaluation of real-time TaqMan® RT-PCR assays for the detection of avian nephritis virus and chicken astrovirus in chickens [J].

Smyth, V. J. ;

Jewhurst, H. L. ;

Wilkinson, D. S. ;

Adair, B. M. ;

Gordon, A. W. ;

Todd, D. .

AVIAN PATHOLOGY, 2010, 39 (06) :467-474

[38] A comparison of the accuracy of the CapitalBio Mycobacterium real-time polymerase chain reaction and the Xpert MTB/RIF assay for the diagnosis of tuberculous meningitis [J].

Sun, Lihua ;

Yao, Liwei ;

Fu, Genlian ;

Lin, Lihua ;

Zhu, Enlan ;

Huang, Jinpeng .

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2021, 104 :92-96

[39] Comparison of four DNA extraction methods for detecting Mycobacterium tuberculosis by real-time PCR and its clinical application in pulmonary tuberculosis [J].

Pan, Shiyang ;

Gu, Bing ;

Wang, Hong ;

Yan, Zihe ;

Wang, Peng ;

Pei, Hao ;

Xie, Weiping ;

Chen, Dan ;

Liu, Genyan .

JOURNAL OF THORACIC DISEASE, 2013, 5 (03) :251-257

[40] Fingerprinting of Mycobacterium tuberculosis isolates by MIRU-VNTR genotyping and detection of isoniazid resistance by real-time PCR [J].

Gaballah, Ahmed ;

Ghazal, Abeer ;

Almiry, Reda ;

Hussein, Somaya ;

Emad, Rasha ;

El-Sherbini, Eglal .

JOURNAL OF MEDICAL MICROBIOLOGY, 2022, 71 (10)

← 1 2 3 4 5 →