Diagnostic accuracy comparison of the Fluorion MTBC QLP 2.1 real-time PCR and the COBAS TaqMan MTB assays for Mycobacterium tuberculosis detection

被引:0
作者
Madihi, Salma [1 ]
Aainouss, Achraf [2 ]
El Messaoudi, Mly Driss [2 ]
Benani, Abdelouaheb [1 ]
机构
[1] Inst Pasteur Maroc, Mol Biol Lab, Pl Louis Pasteur, Casablanca 20360, Morocco
[2] Inst Pasteur Maroc, Lab Mycobacteria & TB, Casablanca, Morocco
关键词
COBAS TaqMan MTB; Diagnosis; Fluorion MTBC QLP 2.1 real-time PCR; Methods comparison; Mycobacterium tuberculosis;
D O I
10.1016/j.mimet.2025.107115
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The Fluorion MTBC QLP 2.1 Real-Time PCR test is a valuable alternative for tuberculosis diagnosis, demonstrating 100 % specificity and 87.5 % sensitivity for respiratory specimens, 97.8 % specificity and 85.3 sensitivity for non-respiratory specimens, and an overall agreement of 92.3 % with the COBAS TaqMan MTB assay.
引用
收藏
页数:3
相关论文
共 50 条
[21]   Is Real-Time PCR Better than Conventional PCR for Mycobacterium tuberculosis Complex Detection in Clinical Samples? [J].
Tortoli, Enrico ;
Urbano, Pasquale ;
Marcelli, Fiorella ;
Simonetti, Tullia M. ;
Cirillo, Daniela M. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (08) :2810-2813
[22]   DIFFERENTIATION OF SARCOIDOSIS FROM TUBERCULOSIS USING REAL-TIME PCR ASSAY FOR THE DETECTION AND QUANTIFICATION OF MYCOBACTERIUM TUBERCULOSIS [J].
Zhou, Y. ;
Li, H. P. ;
Li, Q. H. ;
Zheng, H. ;
Zhang, R. X. ;
Chen, G. ;
Baughman, R. P. .
SARCOIDOSIS VASCULITIS AND DIFFUSE LUNG DISEASES, 2008, 25 (02) :93-99
[23]   Comparison of Diagnostic Performance of Three Real-Time PCR Kits for Detecting Mycobacterium Species [J].
Cho, Sun Young ;
Kim, Min Jin ;
Suh, Jin-Tae ;
Lee, Hee Joo .
YONSEI MEDICAL JOURNAL, 2011, 52 (02) :301-306
[24]   Detection of Mycobacteria, Mycobacterium avium Subspecies, and Mycobacterium tuberculosis Complex by a Novel Tetraplex Real-Time PCR Assay [J].
Sevilla, Iker A. ;
Molina, Elena ;
Elguezabal, Natalia ;
Perez, Valentin ;
Garrido, Joseba M. ;
Justea, Ramn A. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2015, 53 (03) :930-940
[25]   Comparison of two in-house real-time PCR assays with MTB Q-PCR Alert and GenoType MTBDRplus for the rapid detection of mycobacteria in clinical specimens [J].
Seagar, Amie-Louise ;
Neish, Barry ;
Laurenson, Ian F. .
JOURNAL OF MEDICAL MICROBIOLOGY, 2012, 61 (10) :1459-1464
[26]   Evaluation of Propidium Monoazide Real-Time PCR for Early Detection of Viable Mycobacterium tuberculosis in Clinical Respiratory Specimens [J].
Kim, Young Jin ;
Lee, Sun Min ;
Park, Byung Kyu ;
Kim, Sung Soo ;
Yi, Jongyoun ;
Kim, Hyung Hoi ;
Lee, Eun Yup ;
Chang, Chulhun Ludgerus .
ANNALS OF LABORATORY MEDICINE, 2014, 34 (03) :203-209
[27]   A comparison of three real-time PCR assays for the confirmation of Neisseria gonorrhoeae following detection of N-gonorrhoeae using Roche COBAS AMPLICOR [J].
Chui, L. ;
Chiu, T. ;
Kakulphimp, J. ;
Tyrrell, G. J. .
CLINICAL MICROBIOLOGY AND INFECTION, 2008, 14 (05) :473-479
[28]   Conventional and real-time PCR targeting 165 ribosomal RNA for the detection of Mycobacterium tuberculosis complex [J].
Choi, Y. ;
Hong, S-R. ;
Jeon, B-Y. ;
Wang, H-Y. ;
Lee, G-S. ;
Cho, S-N. ;
Shim, T. S. ;
Lee, H. .
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE, 2015, 19 (09) :1102-1108
[29]   Comparison of Six Real-Time PCR Assays for Qualitative Detection of Cytomegalovirus in Clinical Specimens [J].
Binnicker, M. J. ;
Espy, M. E. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (11) :3749-3752
[30]   Detection of Clostridium difficile in animals: comparison of real-time PCR assays with the culture method [J].
Avbersek, Jana ;
Cotman, Marko ;
Ocepek, Matjaz .
JOURNAL OF MEDICAL MICROBIOLOGY, 2011, 60 (08) :1119-1125