Protocol for evaluating humoral immune responses in mice following SARS-CoV-2 vaccination

被引：0
|
作者：
Zhang, Yao [1 ,2 ]
Li, Shixiong [1 ,3 ]
Yu, Jingyou [1 ,4 ]
机构：
[1] Guangzhou Natl Lab, Guangzhou 510005, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Zhongshan Sch Med, Guangzhou 510080, Guangdong, Peoples R China
[3] Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China
[4] Guangzhou Med Univ, Affiliated Hosp 1, State Key Lab Resp Dis, Guangzhou 510182, Guangdong, Peoples R China
来源：
STAR PROTOCOLS | 2025年 / 6卷 / 01期
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1016/j.xpro.2024.103548
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Binding and neutralizing antibodies are critical indicators of protection against viral pathogens and are essential for assessing the immunogenicity and efficacy of a vaccine. Here, we present a protocol comprising two assays for measuring the spike-specific binding and neutralizing antibodies in mouse plasma following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination. We describe steps for determining binding antibody titers using enzyme-linked immunosorbent assay (ELISA) and assessing neutralizing antibody titers through a pseudovirus neutralization assay. For complete details on the use and execution of this protocol, please refer to Jingyou Yu et al.1
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页数：19
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