Characterization and performance of efficient heterotrophic nitrification and aerobic denitrification by Comamonas testosteroni HR5 under low temperature and high alkalinity

被引：0

作者：

Huo, Rui ^{[1
]}

Li, Wanying ^{[1
]}

Di, Yiling ^{[1
]}

Zhou, Shilei ^{[1
]}

机构：

[1] Hebei Univ Sci & Technol, Sch Environm Sci & Engn, Pollut Prevent Biotechnol Lab Hebei Prov, Shijiazhuang 050018, Peoples R China

来源：

JOURNAL OF WATER PROCESS ENGINEERING | 2025年 / 72卷

基金：

中国国家自然科学基金;

关键词：

Heterotrophic nitrification and aerobic denitrification (HN-AD); Comamonas testosteroni; Nitrogen removal; Cold resistance; Alkalinity resistance; NITROGEN REMOVAL; NITRITE ACCUMULATION; ESCHERICHIA-COLI; BACTERIUM; AMMONIUM; ASSIMILATION; ADAPTATION; SYSTEM; NA+;

D O I：

10.1016/j.jwpe.2025.107474

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A newly isolated Comamonas testosteroni strain, HR5, exhibited efficient nitrogen removal capacities at low temperature and high alkalinity. HR5 efficiently removed nitrate nitrogen (removal efficiency >= 95 %) at C/N = 12-15, pH 6-11, and 5-25 degrees C. Furthermore, the removal efficiency of total dissolved nitrogen (TDN) for ammonia, nitrate, and nitrite as the sole nitrogen source system reached 97.90 +/- 0.02 %, 95.16 +/- 0.01 %, and 99.95 +/- 0.00 %, respectively; the removal efficiency for the mixed nitrogen system was >91 % at 5 degrees C and pH 10. Nitrogen balance indicated that HR5 converted initial nitrogen into gaseous products (20.87-77.13 %) and biological nitrogen (19.58-75.65 %), with the percentage of gaseous N increasing as the temperature increased. Furthermore, typical cold-resistance genes (cspA, infB, and rbfA) and alkalinity-resistance genes (Pha and Trk) were involved. These results provide a reference for practical applications of Comamonas testosteroni that involve low temperature and high alkalinity.

引用

页数：11