A Coumarin-Based Reversible Fluorescent Probe for Quantitative Detection and Monitoring of Cellular Glutathione

被引：0
作者：
Wang, Shanshan [1 ]
Yu, Shihua [2 ]
Liu, Zhigang [1 ]
Zeng, Xiaodan [1 ]
机构：
[1] Jilin Inst Chem Technol, Ctr Characterizat & Anal, Jilin, Peoples R China
[2] Jilin Inst Chem Technol, Sch Chem & Pharmaceut Engn, Jilin, Peoples R China
来源：
CHEMISTRYSELECT | 2025年 / 10卷 / 12期
基金：
中国国家自然科学基金;
关键词：
Coumarin; Fluorescence imaging; Glutathione; Reversible fluorescent probe; LIVING CELLS; GSH; CYSTEINE;
D O I：
10.1002/slct.202500218
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
An "on-off-on" reversible fluorescent probe for reversible response to GSH and H2O2 and determination for GSH was designed and synthesized. Considering the important role of GSH in biological system and commonly applied recognition groups, the C & boxH;C bond in coumarin dye was successfully used as the recognition site. This probe showed fast response (within 5 s) and high sensitivity (12.6 nM) in the recognition process. It could be used not only for quantitative determination of GSH, but also could be used for the fluorescence imaging of GSH in cells. In addition, the probe showed reversible fluorescence response for GSH and H2O2, which could be repeated at least 3 times. The experimental results proved that the probe was successfully modified from coumarin and the probe recognized for GSH with significant results.
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页数：8
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