Oxidative stress and filtration responses in Atlantic ribbed mussels (Geukensia demissa) exposed to radium-226

Bivalves have been extensively utilized as sentinel biomonitoring species, organisms used to predict the extent and severity of environmental contamination. However, significant knowledge gaps remain regarding the operationalization and application of indicator species for radioactive contaminants in the marine environment. Dose-dependent organism responses with validated and practical measurement protocols need to be identified for use within biomonitoring frameworks. Our study explored tissue-specific oxidative stress and filtration responses in Geukensia demissa (Atlantic ribbed mussel) following static renewal exposure to a range of aqueous radium-226 (226Ra) concentrations and exposure durations. We investigated a two-tier antioxidant response system, with radical scavenging activity as the primary response and glutathione S-transferase and lipid peroxidation activities as secondary, downstream responses. A first-stage response of 226Ra exposure was observed, indicated by increased radical scavenging activity in the mantle tissues of mussels exposed to the highest treatment concentration (200 nCi/L = 200 ng/L, 73%) compared with the tissues of control and lower concentration (1, 10, and 100 nCi/L = 1, 10, and 100 ng/L) mussels (18%-44%). However, there was no clear impact for the second-stage responses. A reduction in filtration, quantified via algal removal, was also observed for mussels exposed to the highest 226Ra treatment concentration. This work represents the first investigation on the effects of a marine bivalve exposed to aqueous 226Ra. The responses of bivalves to radiological marine pollution, and the potential for cascading impacts to populations and ecosystems, is still relatively unknown but has important implications for ecological and human well-being.