Serum-Derived Exosomal TBX2-AS1 Exacerbates COPD by Altering the M1/M2 Ratio of Macrophages through Regulating the miR-423-5p/miR-23b-3p Axis

被引：0

作者：

Wang, Jinhai ^{[1
]}

Luo, Qing ^{[2
]}

Gu, Tijun ^{[1
]}

An, Fenqin ^{[3
]}

Zhou, Yunzheng ^{[3
]}

Min, Yeping ^{[1
]}

Zhang, Ruiren ^{[3
]}

Jiang, Yiming ^{[1
,3
]}

机构：

[1] Nanjing Med Univ, Affiliated Changzhou Peoples Hosp 2, Dept Emergency, Changzhou 213003, Peoples R China

[2] Peoples Hosp Hainan Tibetan Autonomous Prefecture, Dept Internal Med, Qinghai, Hainan Tibetan, Peoples R China

[3] Peoples Hosp Hainan Tibetan Autonomous Prefecture, Dept Emergency, Qinghai, Hainan Tibetan, Peoples R China

来源：

IMMUNOLOGICAL INVESTIGATIONS | 2025年 / 54卷 / 02期

关键词：

COPD; macrophage polarization; miR-423-5p/miR-23b-3p; serum exosomes; TBX2-AS1; OBSTRUCTIVE PULMONARY-DISEASE; INFLAMMATION;

D O I：

10.1080/08820139.2024.2434692

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

ObjectiveTo investigate the mechanism of serum exosomes in chronic obstructive pulmonary disease (COPD), especially the effect of lncRNA TBX2-AS1 on macrophage polarization.MethodsScreen differentially expressed genes through bioinformatics analysis, detect the expression of related molecules in clinical samples and cell experiments, construct a mouse model and conduct functional rescue experiments, using various experimental techniques such as RT - qPCR, Western Blot, flow cytometry, ELISA, and luciferase reporter assay.ResultsTBX2-AS1 is highly expressed in the serum and serum exosomes of COPD patients, and it can promote macrophage M1 polarization and inhibit M2 polarization; it exerts its role by negatively regulating the miR-423-5p/miR-23b - 3p axis, where miR-423-5p inhibits CELSR2 expression to prevent M1 polarization, and miR-23b-3p inhibits NEK6 expression to promote M2 polarization; in vivo experiments, down-regulation of CELSR2/NEK6 can reverse the promoting effect of COPD serum exosomes on lung injury and inflammation.ConclusionCOPD serum exosomes deliver TBX2-AS1 to macrophages, regulate the miR-423-5p-CELSR2/miR-23b-3p-NEK6 pathway, affect macrophage polarization, and exacerbate the progression of COPD, providing new directions and potential targets for the diagnosis and treatment of COPD.

引用

页码：271 / 295

页数：25