The protective roles of boron against copper excess in citrus roots: Insights from physiology, transcriptome, and metabolome

被引:0
作者
Chen, Xu-Feng [1 ]
Huang, Wei-Tao [1 ]
Shen, Qian [1 ]
Huang, Wei-Lin [1 ]
Lu, Fei [1 ]
Yang, Lin-Tong [1 ]
Lai, Ning-Wei [1 ]
Huang, Zeng-Rong [1 ]
Chen, Li-Song [1 ]
机构
[1] Fujian Agr & Forestry Univ, Coll Resources & Environm, 15 Shangxiadian Rd, Fuzhou 350002, Peoples R China
关键词
Cell wall; Methylglyoxal; Primary metabolism; Reactive oxygen species; Secondary metabolism; RICE ORYZA-SATIVA; OXIDATIVE STRESS; CARBOHYDRATE-METABOLISM; ARABIDOPSIS; TOLERANCE; MEMBRANES; L; BIOSYNTHESIS; ACCUMULATION; EXPRESSION;
D O I
10.1016/j.plaphy.2025.109588
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Boron (B) deficiency and copper (Cu) excess are common problems in citrus orchard soils. Citrus sinensis seedlings were exposed to 25 (B25) or 2.5 (B2.5) mu M H3BO3 and 0.5 (Cu0.5) or 350 (Cu350) mu M CuCl3 for 24 weeks. Cu350 upregulated 2210 (1012) genes and 482 (341) metabolites and downregulated 3201 (695) genes and 175 (43) metabolites in roots at B2.5 (B25). Further analysis showed that the B-mediated mitigation of Cu toxicity in roots involved the coordination of the following aspects: (a) enhancing the ability to maintain cell wall and plasma membrane stability and function; (b) lowering the impairment of Cu350 to primary and secondary metabolisms and enhancing their adaptability to Cu350; and (c) alleviating Cu350-induced oxidative stress via the coordination of reactive oxygen species (ROS) and methylglyoxal detoxification systems. Cu350 upregulated the abundances of some saccharides, amino acids and derivatives, phospholipids, secondary metabolites, and vitamins, and the expression of several ROS detoxification-related genes in roots of B2.5-treated seedlings (RB2.5), but these adaptive responses did not prevent RB2.5 from Cu-toxicity (oxidative damage). The study identified some genes, metabolites, and metabolic processes/pathways possibly involved in root Cu tolerance. Additionally, the responses of gene expression and metabolite profiling to Cu-B treatments differed between leaves and roots. Therefore, this study provided novel information for B to reduce Cu toxicity in roots and might contribute to the development of soil amendments targeting Cu excess in citrus and other crops.
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页数:19
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共 95 条
  • [41] Kidwai M., Dhar Y.V., Gautam N., Tiwari M., Ahmad I.Z., Asif M.H., Chakrabarty D., Oryza sativa class III peroxidase (OsPRX38) overexpression in Arabidopsis thaliana reduces arsenic accumulation due to apoplastic lignification, J. Hazard. Mater., 362, pp. 383-393, (2019)
  • [42] Kim W.S., Sun-Hyung J., Oehrle N.W., Jez J.M., Krishnan H.B., Overexpression of ATP sulfurylase improves the sulfur amino acid content, enhances the accumulation of Bowman-Birk protease inhibitor and suppresses the accumulation of the β-subunit of β-conglycinin in soybean seeds, Sci. Rep., 10, (2020)
  • [43] Kocaman A., Combined interactions of amino acids and organic acids in heavy metal binding in plants, Plant Signal. Behav., 18, (2023)
  • [44] Kovacik J., Dresler S., Peterkova V., Babula P., Metal-induced oxidative stress in terrestrial macrolichens, Chemosphere, 203, pp. 402-409, (2018)
  • [45] Landa P., Dytrych P., Prerostova S., Petrova S., Vankova R., Vanek T., Transcriptomic response of Arabidopsis thaliana exposed to CuO nanoparticles, bulk material, and ionic copper, Environ. Sci. Technol., 51, pp. 10814-10824, (2017)
  • [46] Li Y., Han M.-Q., Lin F., Ten Y., Lin J., Zhu D.-H., Guo P., Weng Y.-B., Chen L.-S., Soil chemical properties, ‘Guanximiyou’ pummelo leaf mineral nutrient status and fruit quality in the southern region of Fujian province, J. Soil Sci. Plant Nutr., 15, pp. 615-628, (2015)
  • [47] Li Q., Chen H.-H., Qi Y.-P., Ye X., Yang L.-T., Huang Z.-R., Chen L.-S., Excess copper effects on growth, uptake of water and nutrients, carbohydrates, and PSII photochemistry revealed by OJIP transients in Citrus seedlings, Environ. Sci. Pollut. Res., 26, pp. 30188-30205, (2019)
  • [48] Li S., Zhang K., Tian J., Chang K., Yuan S., Zhou Y., Zhao H., Zhong F., Fulvic acid mitigates cadmium toxicity-induced damage in cucumber seedlings through the coordinated interaction of antioxidant enzymes, organic acid, and amino acid, Environ. Sci. Pollut. Res., 30, pp. 28780-28790, (2023)
  • [49] Lim J.D., Hahn S.J., Yu C.Y., Chung I.M., Expression of the glutathione S-transferase gene (NT107) in transgenic Dianthus superbus, Plant Cell Tissue Organ Cult, 80, pp. 277-286, (2005)
  • [50] Liman R., Ali M.M., Cigerci I.H., Istifli E.S., Sarikurkcu C., Cytotoxic and genotoxic evaluation of copper oxychloride through Allium test and molecular docking studies, Environ. Sci. Pollut. Res., 28, 33, pp. 44998-45008, (2021)