Removal of antibiotic resistance genes and co-selectors in a full-scale sewage treatment plant during droughts and floods

被引:0
作者
Dixit, Sourabh [1 ]
Vijayan, Jayavignesh [2 ,3 ]
Singh, Harshita [1 ]
Nambi, Indumathi M. [2 ,3 ]
Renganathan, T. [2 ,3 ]
Ravikrishna, R. [2 ,3 ]
Singh, Gargi [1 ]
机构
[1] Indian Inst Technol, Dept Civil Engn, Roorkee 247667, Uttarakhand, India
[2] Indian Inst Technol, Dept Civil Engn, Madras 600036, Tamil Nadu, India
[3] Indian Inst Technol, Dept Chem Engn, Madras 600036, Tamil Nadu, India
关键词
MUNICIPAL WASTE-WATER; CLASS; INTEGRONS; ESCHERICHIA-COLI; ANTIMICROBIAL RESISTANCE; ANAEROBIC-DIGESTION; FATE; PREVALENCE; COMMUNITY; VIRULENCE; BACTERIA;
D O I
10.1039/d4ew00875h
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
We investigated the effect of droughts and floods on the removal of antibiotic resistance genes and co-selectors in a 60 MLD sewage treatment plant (STP) in Perungudi town, Chennai city. Samples were collected once a month for 13 months and analysed for 7 antibiotic resistance genes (sul1, ermF, tetW, mcr5, sul2, parC, and bla(OXA-1)), the most used antibiotic in the region (ciprofloxacin), a non-pharmaceutical antibiotic (triclosan), and 3 co-selective heavy metals (copper, chromium, and lead). It was found that the total bacterial count decreased significantly in the STP (p-value = 0.03, Wilcoxon rank sum test), but the relative abundance of ARGs was not reduced in the STP. In fact, the relative abundances of tetW and bla(OXA-1) increased in the maturation pond and were higher in the effluent than in the influent (p-value = 0.03, Wilcoxon rank sum test). On the other hand, the relative abundances of most ARGs were reduced significantly in the anaerobic digester (p-value < 0.05, Wilcoxon rank sum test). Overall relative abundances of mcr5, tetW, ermF, intI1, and sul2 in the raw sewage were higher in the dry summer than in the monsoon. The relative abundance of intI1 was correlated (Spearman correlation) with the relative abundances of mcr5 (rho = 0.54), ermF (rho = 0.52), and tetW (rho = 0.61). Except for chromium, all the other targeted co-selectors were significantly removed from the STP throughout the year (p > 0.05, Wilcoxon rank sum test). Overall, the targeted ARGs were not removed in the STP, and this underperformance was unaffected by periods of droughts and floods.
引用
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页码:702 / 713
页数:12
相关论文
共 84 条
[81]  
Wickham H., 2016, ggpolt2 Elegant Graphics for Data Analysis. Use R! series
[82]   Occurrence and removal of antibiotics, antibiotic resistance genes, and bacterial communities in hospital wastewater [J].
Yao, Shijie ;
Ye, Jianfeng ;
Yang, Qing ;
Hu, Yaru ;
Zhang, Tianyang ;
Jiang, Lei ;
Munezero, Salvator ;
Lin, Kuangfei ;
Cui, Changzheng .
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2021, 28 (40) :57321-57333
[83]   Antibiotic resistance genes and intI1 prevalence in a swine wastewater treatment plant and correlation with metal resistance, bacterial community and wasteWater parameters [J].
Yuan, Qing-Bin ;
Zhai, Yi-Fan ;
Mao, Bu-Yun ;
Hu, Nan .
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2018, 161 :251-259
[84]   Metagenomics highlights the impact of climate and human activities on antibiotic resistance genes in China's estuaries [J].
Zheng, Dongsheng ;
Yin, Guoyu ;
Liu, Min ;
Hou, Lijun ;
Yang, Yi ;
Liu, Xinran ;
Jiang, Yinghui ;
Chen, Cheng ;
Wu, Han .
ENVIRONMENTAL POLLUTION, 2022, 301