Antidepressants as new endocrine disruptors? - transcriptomic profiling in gonads of Mytilus trossulus exposed to norfluoxetine

被引：0

作者：

Gozdzik, Paulina ^{[1
]}

Smolarz, Katarzyna ^{[2
]}

Hallmann, Anna ^{[1
]}

机构：

[1] Med Univ Gdansk, Dept Pharmaceut Biochem, Debinki 1, PL-80211 Gdansk, Poland

[2] Univ Gdansk, Dept Marine Ecosyst Functioning, Marszalka Pilsudskiego 46, PL-81378 Gdynia, Poland

来源：

MARINE POLLUTION BULLETIN | 2024年 / 208卷

关键词：

Antidepressants; Transcriptomic profiling; Blue mussel; CYP450; PRIMARY CILIARY DYSKINESIA; FLUOXETINE; SEROTONIN; IDENTIFICATION; EXPRESSION; COMPLEX; GENES; IMMUNOLOCALIZATION; MUTATIONS; PROTEINS;

D O I：

10.1016/j.marpolbul.2024.117015

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

In this study an impact of norfluoxetine (NFLU) on Mytilus trossulus gonads was investigated focusing on sexrelated differences in hormonal changes, gene expression, and transcriptomic profiling. Sex-specific differences in gonadal serotonin levels were found. NFLU stimulates serotonin synthesis and/or transport in female gonads, potentially accelerating oocyte maturation and gamete release. In males, NFLU decreases serotonin level what likely leads to impeding sperm maturation and thus spawning delay. Transcriptomic analyses highlighted the presence of NFLU-induced changes in gene expression related to gametogenesis and neurotransmission. In females, NFLU upregulated genes associated with oocyte development and downregulated those involved in sperm maturation. NFLU-treated males exhibited mixed effects in their genes in relation to spermatogenesis. Additionally, sex-related differences in the expression of the CYP450 genes responsible for detoxification were found. Overall, norfluoxetine acts as an endocrine-disrupting chemical and impacts gonadal serotonin levels and gene expression, potentially disrupting reproductive success of M. trossulus.

引用

页数：14

共 75 条

[1]

Alavi S., 2017, Serotonin-A Chemical Messenger Between All Types of Living Cells, DOI [10.5772/intechopen.69165, DOI 10.5772/INTECHOPEN.69165]

[2] CLINICAL PHARMACOKINETICS OF FLUOXETINE [J].

ALTAMURA, AC ;

MORO, AR ;

PERCUDANI, M .

CLINICAL PHARMACOKINETICS, 1994, 26 (03) :201-214

[3]

Andrews S, 2010, FastQC: A quality control tool for high throughput sequence data

[4] Immunolocalization of cubilin, megalin, apolipoprotein J, and apolipoprotein A-I in the uterus and oviduct [J].

Argraves, WS ;

Morales, CR .

MOLECULAR REPRODUCTION AND DEVELOPMENT, 2004, 69 (04) :419-427

[5] GLUTAMATE, GABA, GLYCINE AND TAURINE MODULATE SEROTONIN SYNTHESIS AND RELEASE IN ROSTRAL AND CAUDAL RHOMBENCEPHALIC RAPHE CELLS IN PRIMARY CULTURES [J].

BECQUET, D ;

HERY, M ;

FRANCOISBELLAN, AM ;

GIRAUD, P ;

DEPREZ, P ;

FAUDON, M ;

FACHE, MP ;

HERY, F .

NEUROCHEMISTRY INTERNATIONAL, 1993, 23 (03) :269-283

[6] TIGAR, a p53-inducible regulator of glycolysis and apoptosis [J].

Bensaad, Karim ;

Tsuruta, Atsushi ;

Selak, Mary A. ;

Calvo Vidal, M. Nieves ;

Nakano, Katsunori ;

Bartrons, Ramon ;

Gottlieb, Eyal ;

Vousden, Karen H. .

CELL, 2006, 126 (01) :107-120

[7] The antidepressant fluoxetine alters mechanisms of pre- and post-copulatory sexual selection in the eastern mosquitofish (Gambusia holbrooki) [J].

Bertram, Michael G. ;

Ecker, Tiarne E. ;

Wong, Bob B. M. ;

O'Bryan, Moira K. ;

Baumgartner, John B. ;

Martin, Jake M. ;

Saaristo, Minna .

ENVIRONMENTAL POLLUTION, 2018, 238 :238-247

[8] Trimmomatic: a flexible trimmer for Illumina sequence data [J].

Bolger, Anthony M. ;

Lohse, Marc ;

Usadel, Bjoern .

BIOINFORMATICS, 2014, 30 (15) :2114-2120

[9] A Tissue-Mapped Axolotl De Novo Transcriptome Enables Identification of Limb Regeneration Factors [J].

Bryant, Donald M. ;

Johnson, Kimberly ;

DiTommaso, Tia ;

Tickle, Timothy ;

Couger, Matthew Brian ;

Payzin-Dogru, Duygu ;

Lee, Tae J. ;

Leigh, Nicholas D. ;

Kuo, Tzu-Hsing ;

Davis, Francis G. ;

Bateman, Joel ;

Bryant, Sevara ;

Guzikowski, Anna R. ;

Tsai, Stephanie L. ;

Coyne, Steven ;

Ye, William W. ;

Freeman, Robert M., Jr. ;

Peshkin, Leonid ;

Tabin, Clifford J. ;

Regev, Aviv ;

Haas, Brian J. ;

Whited, Jessica L. .

CELL REPORTS, 2017, 18 (03) :762-776

[10] Psychiatric pharmaceuticals in the environment [J].

Calisto, Vania ;

Esteves, Valdemar I. .

CHEMOSPHERE, 2009, 77 (10) :1257-1274

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