Genome-wide analysis of the laccase gene family in Arachis hypogaea and functional characterization of AhLAC63 involved in lignin biosynthesis and abiotic stress

Plant laccases (LACs) play a vital role in lignification and participate in multiple biotic/abiotic stress responses. However, little is known about their role in lignin deposition and stress resistance in cultivated peanut (Arachis hypogaea L.). In this study, 80 putative peanut laccase genes (AhLACs) were identified and clustered into seven distinct phylogenetic groups. While the AhLAC members of group VI were lost, a novel specific group VIII was discovered in peanut. AhLACs within same group generally have similar gene structures and protein motif organizations. Expression pattern and subcellular cellular analysis revealed that AhLAC63 is a candidate gene involved in lignification and abiotic stress response. In addition, introducing AhLAC63 into the Arabidopsis laccase mutant (lac4 lac11) restored its lignin contents and abiotic stress tolerance. Moreover, the overexpression of AhLAC63 significantly altered phenylpropanoid metabolism flux and increased lignin content in peanut hairy roots. This study not only enables the further exploration of LAC biological functions in peanut, but also provides new gene resources for improving stress resistance in crops.