Cell Culture of a Swine Genotype 4 Hepatitis E Virus Strain

被引：1

作者：

Gong, Ga ^{[1
]}

Xin, Jiaojiao ^{[1
]}

Lou, Yongzhi ^{[1
]}

Qiong, Da ^{[1
]}

Dawa, Zhuoma ^{[2
]}

Gesang, Zhuoma ^{[3
]}

Suolang, Sizhu ^{[1
]}

机构：

[1] Xizang Agr & Anim Husb Univ, Anim Sci Coll, Nyingchi, Peoples R China

[2] Vet Stn, Lhasa, Peoples R China

[3] Anim Dis Prevent & Control Ctr Xizang Autonomous R, Lhasa, Peoples R China

来源：

JOURNAL OF MEDICAL VIROLOGY | 2024年 / 96卷 / 11期

关键词：

cell culture; GT4; hepatitis E virus; swine;

D O I：

10.1002/jmv.70031

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

HEV infection has become a global health concern. The study of HEV pathogenicity has been hindered by the lack of a suitable in vitro culture system. In the present research, we systematic demonstration of efficient replication of swine GT4 HEV in A549 cells, Huh-7 cells, and HepG2/C3A cell lines. The results of the immunofluorescence assay and immunofluorescence confocal assay showed that swine GT4 HEV is efficiently replicated in three cell lines at 72 h postinoculation. Meanwhile, we also detected the virus titer quantified were increased at 2-, 6,- and 11-days postinoculation. Moreover, we successfully observed HEV virus particles in the cell suspension at 6 days postinoculation. This finding holds significance for advancing in vitro HEV studies.

引用

页数：6

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