Metagenomic Next-Generation Sequencing in the Diagnosis of Pulmonary Infections after Allogeneic Hematopoietic Stem Cell Transplantation

被引：1

作者：

Fu, Rong ^{[1
]}

Xu, Jun ^{[1
]}

Fan, Zhiping ^{[1
]}

Qu, Hong ^{[2
]}

Jiang, Yirong ^{[3
]}

Xiong, Wenjie ^{[4
]}

Huang, Fen ^{[1
]}

Xuan, Li ^{[1
]}

Xu, Na ^{[1
]}

Liu, Hui ^{[1
]}

Wang, Zhixiang ^{[1
]}

Sun, Jing ^{[1
]}

Liu, Qifa ^{[1
]}

Lin, Ren ^{[1
]}

机构：

[1] Southern Med Univ, Nanfang Hosp, Clin Med Res Ctr Hematol Dis Guangdong Prov, Dept Hematol, Guangzhou 510515, Peoples R China

[2] Guangzhou Med Univ, Affiliated Panyu Cent Hosp, Infect Med Res Inst Panyu Dist, Guangzhou, Peoples R China

[3] Dongguan Peoples Hosp, Dept Otorhinolaryngol, Dongguan, Peoples R China

[4] Foresea Life Insurance Guangzhou Gen Hosp, Guangzhou, Peoples R China

来源：

TRANSPLANTATION AND CELLULAR THERAPY | 2024年 / 30卷 / 11期

基金：

中国国家自然科学基金;

关键词：

Metagenomic next-generation sequencing; Allogeneic hematopoietic stem cell transplantation; Pulmonary infection; Conventional microbiological tests; Bronchoalveolar lavage fluid; PATHOGEN DETECTION; PNEUMONIA; UTILITY;

D O I：

10.1016/j.jtct.2024.08.014

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Early and accurate identification of pathogens in pulmonary infections after allogeneic hematopoietic stem cell transplantation (allo-HSCT) is critically important. The clinical usefulness of metagenomic next-generation sequencing (mNGS) in the diagnosis of pulmonary infections after allo-HSCT remains under discussion. This multicenter retrospective study was conducted to compare mNGS and conventional microbiological tests (CMTs) in identifying the pathogens of pulmonary infections in allo-HSCT recipients. One hundred forty alloHSCT recipients with suspected pulmonary infections who underwent bronchoscopy were included. mNGS and CMTs performed on bronchoalveolar lavage fluid specimens showed 71.4% positivity on mNGS compared to 55.0% positivity on CMTs. mNGS identified 182 pathogens, including bacteria (n = 88), fungi (n = 35) and viruses (n = 59), compared to 106 pathogens detected by CMTs (bacteria, n = 31; fungi, n = 24; viruses, n = 51). Pulmonary infection was finally diagnosed in 98 patients, including 22 bacterial, 7 fungal, 18 viral, and 48 mixed infections and 3 infections with an unknown pathogen. Mixed infections were identified in 50.5% of the patients with pulmonary infection. The sensitivity of mNGS and CMTs for diagnosing pulmonary infections was 88.8% and 69.4%, respectively (P = .001), and the specificity were 81.0% and 85.7%, respectively (P = .688). Our findings suggest that mNGS may be a promising technology for diagnosing pulmonary infections in allo-HSCT recipients. (c) 2024 The American Society for Transplantation and Cellular Therapy. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.

引用

页码：1110e1 / 1110e10

页数：10