In-cell NMR spectroscopy of nucleic acids: Basic concepts, practical aspects, and applications

被引:0
|
作者
Foldynova-Trantirkova, Silvie [1 ]
Harnos, Jakub [2 ]
Rynes, Jan [1 ]
Zlinska, Vladimira [1 ,3 ]
Trantirek, Lukas [1 ]
机构
[1] Masaryk Univ, Cent European Inst Technol, Kamenice 753-5, Brno 62500, Czech Republic
[2] Masaryk Univ, Fac Sci, Dept Expt Biol, Kamenice 753-5, Brno 62500, Czech Republic
[3] Masaryk Univ, Fac Sci, Natl Ctr Biomol Res, Kamenice 753-5, Brno 62500, Czech Republic
关键词
In-cell NMR; DNA; RNA; Xenopus oocytes; Human cells; Cellular structural biology; REVERSIBLE MEMBRANE PERMEABILIZATION; XENOPUS OOCYTE MATURATION; G-QUADRUPLEX; LIVING CELLS; F-19; NMR; PROGESTERONE-RECEPTOR; PROTEIN INTERACTIONS; TELOMERIC DNA; LAEVIS; RNA;
D O I
10.1016/j.pnmrs.2025.101560
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
In-cell NMR spectroscopy has recently emerged as a unique source of atomically resolved information on the structure, dynamics, and interactions of nucleic acids (NAs) within the intracellular space of living cells. Its recent applications have helped reveal fundamental differences in the behaviour of NAs in cells compared to the in vitro conditions commonly used for their study, as well as in physiologically distinct cellular states. This review covers the fundamental principles and practical aspects of acquiring in-cell NMR data in currently established eukaryotic cellular models, Xenopus laevis oocytes, and human cells. The primary purpose of this review is to present and discuss the technical and conceptual aspects of in-cell NMR sample preparations and their manipulations during in-cell NMR data acquisition, as understanding these aspects is vital for comprehending the physiological significance of in-cell NMR data and the information they provide. Considerations on the planning of in-cell NMR experiments and the presentation of in-cell NMR data on nucleic acids are discussed. We hope this will enable readers to navigate through the ever-growing pool of in-cell NMR literature and gain the knowledge needed to assess and comprehend published data independently. Additionally, we hope it will inspire some readers to actively participate in this rapidly expanding and fascinating field of cellular structural biology.
引用
收藏
页数:31
相关论文
共 50 条
  • [31] In-cell structure determination using paramagnetic NMR spectroscopy
    Su, Xun-Cheng
    Huber, Thomas
    1600, Royal Society of Chemistry (2020-January): : 90 - 102
  • [32] In-Cell NMR Spectroscopy in Protein Chemistry and Drug Discovery
    Ohno, Ayako
    Inomata, Kohsuke
    Tochio, Hidehito
    Shirakawa, Masahiro
    CURRENT TOPICS IN MEDICINAL CHEMISTRY, 2011, 11 (01) : 68 - 73
  • [33] Diffusion NMR and MRI: Basic concepts and applications
    Cohen, Y
    Neeman, M
    ISRAEL JOURNAL OF CHEMISTRY, 2003, 43 (1-2) : I - I
  • [34] APPLICATIONS OF NMR-SPECTROSCOPY TO STUDIES OF REACTIVE INTERMEDIATES AND THEIR INTERACTIONS WITH NUCLEIC-ACIDS
    HARRIS, TM
    STONE, MP
    HARRIS, CM
    CHEMICAL RESEARCH IN TOXICOLOGY, 1988, 1 (02) : 79 - 96
  • [35] Nucleic Acids Electrotransfer In Vivo: Mechanisms and Practical Aspects
    Andre, Franck M.
    Mir, Lluis M.
    CURRENT GENE THERAPY, 2010, 10 (04) : 267 - 280
  • [36] Fluorescence: Basic concepts, practical aspects, and some anecdotes
    Jameson, DM
    Croney, JC
    Moens, PDJ
    BIOPHOTONICS, PT A, 2003, 360 : 1 - 43
  • [37] Basic concepts and recent advances in electrochemical analysis of nucleic acids
    Ferapontova, Elena E.
    CURRENT OPINION IN ELECTROCHEMISTRY, 2017, 5 (01) : 218 - 225
  • [38] Applications of In-Cell NMR in Structural Biology and Drug Discovery
    Kang, CongBao
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20 (01):
  • [39] NMR spectroscopy: from basic concepts to advanced methods
    Luchinat, Enrico
    FROM MOLECULES TO LIVING ORGANISMS: AN INTERPLAY BETWEEN BIOLOGY AND PHYSICS, 2016, : 134 - 168
  • [40] In-cell NMR spectroscopy of proteins inside Xenopus laevis oocytes
    Tomomi Sakai
    Hidehito Tochio
    Takeshi Tenno
    Yutaka Ito
    Tetsuro Kokubo
    Hidekazu Hiroaki
    Masahiro Shirakawa
    Journal of Biomolecular NMR, 2006, 36 : 179 - 188