Development and Application of Pik Locus-Specific Molecular Markers for Blast Resistance Genes in Yunnan Japonica Rice Cultivars

被引：0
作者：
Liu, Pei [1 ]
Zhou, Wumin [1 ,2 ]
Dong, Liying [1 ]
Liu, Shufang [1 ]
Nawaz, Gul [1 ]
Huang, Liyu [2 ]
Yang, Qinzhong [1 ]
机构：
[1] Yunnan Acad Agr Sci, Agr Environm & Resources Inst, Key Lab Green Prevent & Control Agr Transboundary, Kunming 650205, Peoples R China
[2] Yunnan Univ, Sch Agr, Kunming 650504, Peoples R China
来源：
PLANTS-BASEL | 2025年 / 14卷 / 04期
基金：
中国国家自然科学基金;
关键词：
<italic>Oryza sativa</italic> L; <italic>Pik</italic> locus; rice blast; resistance gene; molecular marker; MAGNAPORTHE-ORYZAE; CONFERRING RESISTANCE; DISEASE-RESISTANCE; ASSISTED SELECTION; IDENTIFICATION; ORIGIN; ALLELE; FUNGUS;
D O I：
10.3390/plants14040592
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most devastating diseases affecting rice production worldwide, resulting in significant yield losses and threatening global food security. The severity of rice blast, particularly in susceptible regions, underscores the urgent need for available effective resistance strategies. In this study, six sets of gene-specific molecular markers for the Pik locus associated with rice blast resistance were developed based on publicly available gene sequences. Experimental validation confirmed their high accuracy. During the marker development process, a novel haplotype of the Pik locus was identified. This haplotype is characterized by 14 bp mutations and a 9 bp insertion within the coding sequence region when compared to the Pikh allele. Subsequently, a molecular marker specific to this haplotype was developed and validated. The application of these seven sets of markers to analyze 163 japonica rice cultivars bred in Yunnan Province between 1980 and 2020 revealed that 38.65% of the cultivars carry the Piks allele, indicating a low resistance frequency against the rice blast fungus under field conditions. In contrast, only a small proportion of cultivars possess other Pik locus alleles, which exhibit higher resistance frequencies. These findings highlight the limited utilization of Pik locus genes in japonica rice breeding in Yunnan. Furthermore, 21.47% of the cultivars lack any of the aforementioned Pik locus alleles, indicating the genetic diversity and complexity of the rice genetic resources of Yunnan Province.
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页数：14
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