mRNA encoding antibodies against hemagglutinin and nucleoprotein prevents influenza virus infection in vitro

被引：0

作者：

Zabrodskaya, Y. A. ^{[1
,2
]}

Gavrilova, N. V. ^{[1
,2
]}

Elpaeva, E. A. ^{[1
]}

Lozhkov, A. A. ^{[1
,2
]}

Vysochinskaya, V. V. ^{[1
,2
]}

Dobrovolskaya, O. A. ^{[1
]}

Dovbysh, O. V. ^{[2
]}

Zimmerman, E. L. ^{[1
,2
]}

Dav, P. N. ^{[2
]}

Brodskaia, A. V. ^{[1
,2
]}

Sakhenberg, E. I. ^{[1
]}

Shaldzhyan, A. A. ^{[1
]}

Demaev, A. A. ^{[2
]}

Maslov, M. A. ^{[3
]}

Vasin, A. V. ^{[1
,2
]}

机构：

[1] Russian Minist Hlth, Smorodintsev Res Inst Influenza, Prof Popov St 15-17, St Petersburg 197376, Russia

[2] Peter Great St Petersburg Polytech Univ, Inst Biomed Syst & Biotechnol, Politekhn Skaya 29, St Petersburg 194064, Russia

[3] MIREA Russian Technol Univ, Lomonosov Inst Fine Chem Technol, 86 Vernadsky Ave, Moscow 119571, Russia

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2024年 / 738卷

关键词：

mRNA therapeutics; Antibodies; Fab fragments; Influenza virus; Antivirals;

D O I：

10.1016/j.bbrc.2024.150945

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The emergence of new influenza virus strains presents a continuous challenge for global public health. mRNA technology offers a promising platform for rapidly developing therapeutics, particularly monoclonal antibodies, that can protect against viral infections. In this study, we engineered mRNA constructs encoding two types of antibodies: secreted antibodies specific to the hemagglutinin of the influenza A virus, based on previously characterized Fi6 antibodies, and intracellular Fab fragments targeting the nucleoprotein of the influenza B virus, derived from the 2/3 antibodies. The administration of mRNA constructs in vitro resulted in the successful synthesis of functional antibodies, which exhibited antiviral activity against influenza viruses. This study confirms the feasibility of using mRNA technology to develop therapeutic antibodies against influenza virus infections. The findings pave the way for future clinical applications of mRNA-based therapeutics, enhancing preparedness for emerging viral threats.

引用

页数：6

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