Antibody Affinity Extraction Outperforms Native Digestion as a Sample Preparation Method for LC-MS Detection of Host Cell Proteins in Substances

被引：0

作者：

Zilberman, Alla ^{[1
]}

Bertin, Darren ^{[1
]}

Stahlschmidt, Stephen ^{[1
]}

Barnhill, Johanna ^{[1
]}

Licknack, Timothy ^{[1
]}

Isaac, Jared ^{[1
]}

机构：

[1] Cygnus Technol LLC, Tech Mkt, Leland, NC 28451 USA

来源：

BIOPHARM INTERNATIONAL | 2024年 / 37卷 / 06期

关键词：

MONOCLONAL-ANTIBODIES; SPECTROMETRY; BIOTHERAPEUTICS; QUANTIFICATION; IMPURITIES; HCPS;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

引用

页数：36

共 22 条

[1] Improved host cell protein analysis in monoclonal antibody products through ProteoMiner [J].

Chen, I-Hsuan ;

Xiao, Hui ;

Li, Ning .

ANALYTICAL BIOCHEMISTRY, 2020, 610

[2] Improved Host Cell Protein Analysis in Monoclonal Antibody Products through Molecular Weight Cutoff Enrichment [J].

Chen, I-Hsuan ;

Xiao, Hui ;

Daly, Thomas ;

Li, Ning .

ANALYTICAL CHEMISTRY, 2020, 92 (05) :3751-3757

[3] Enhanced Detection of Low-Abundance Host Cell Protein Impurities in High-Purity Monoclonal Antibodies Down to 1 ppm Using Ion Mobility Mass Spectrometry Coupled with Multidimensional Liquid Chromatography [J].

Doneanu, Catalin E. ;

Anderson, Malcolm ;

Williams, Brad J. ;

Lauber, Matthew A. ;

Chakraborty, Asish ;

Chen, Weibin .

ANALYTICAL CHEMISTRY, 2015, 87 (20) :10283-10291

[4] Analysis of host-cell proteins in biotherapeutic proteins by comprehensive online two-dimensional liquid chromatography/mass spectrometry [J].

Doneanu, Catalin E. ;

Xenopoulos, Alex ;

Fadgen, Keith ;

Murphy, Jim ;

Skilton, St. John ;

Prentice, Holly ;

Stapels, Martha ;

Chen, Weibin .

MABS, 2012, 4 (01) :24-44

[5] Quantitative Host Cell Protein Analysis Using Two Dimensional Data Independent LC-MSE [J].

Farrell, Amy ;

Mittermayr, Stefan ;

Morrissey, Brian ;

Mc Loughlin, Niaobh ;

Iglesias, Natalia Navas ;

Marison, Ian W. ;

Bones, Jonathan .

ANALYTICAL CHEMISTRY, 2015, 87 (18) :9186-9193

[6] Targeted Host Cell Protein Quantification by LC-MRM Enables Biologics Processing and Product Characterization [J].

Gao, Xinliu ;

Rawal, Baibhav ;

Wang, Yi ;

Li, Xuanwen ;

Wylie, David ;

Liu, Yan-Hui ;

Breunig, Lloyd ;

Driscoll, Dennis ;

Wang, Fengqiang ;

Richardson, Douglas D. .

ANALYTICAL CHEMISTRY, 2020, 92 (01) :1007-1015

[7] A Novel Sample Preparation for Shotgun Proteomics Characterization of HCPs in Antibodies [J].

Huang, Lihua ;

Wang, Ning ;

Mitchell, Charles E. ;

Brownlee, Tammy ;

Maple, Steven R. ;

De Felippis, Michael R. .

ANALYTICAL CHEMISTRY, 2017, 89 (10) :5436-5444

[8] Combination of FAIMS, Protein A Depletion, and Native Digest Conditions Enables Deep Proteomic Profiling of Host Cell Proteins in Monoclonal Antibodies [J].

Johnson, Reid O'Brien ;

Greer, Tyler ;

Cejkov, Milos ;

Zheng, Xiaojing ;

Li, Ning .

ANALYTICAL CHEMISTRY, 2020, 92 (15) :10478-10484

[9] Evaluation of Peptide Fractionation and Native Digestion as Two Novel Sample Preparation Workflows to Improve HCP Characterization by LC-MS/MS [J].

Kufer, Regina ;

Haindl, Markus ;

Wegele, Harald ;

Wohlrab, Stefanie .

ANALYTICAL CHEMISTRY, 2019, 91 (15) :9716-9723

[10] Profiling Active Enzymes for Polysorbate Degradation in Biotherapeutics by Activity-Based Protein Profiling [J].

Li, Xuanwen ;

Chandra, Divya ;

Letarte, Simon ;

Adam, Gregory C. ;

Welch, Jonathan ;

Yang, Rong-Sheng ;

Rivera, Shannon ;

Bodea, Smaranda ;

Dow, Alex ;

Chi, An ;

Strulson, Christopher A. ;

Richardson, Douglas D. .

ANALYTICAL CHEMISTRY, 2021, 93 (23) :8161-8169

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