Effect of a modified methyl methacrylate-p-styrene sulfonic acid copolymer-based gel desensitizer on dentin permeability and tubule occlusion in human dentin in vitro

Background/purpose: Tubular occlusion is an effective method to treat dentin hypersensitivity. This study aimed to determine the effect of a modified methyl methacrylate-pstyrene sulfonic acid copolymer-based gel desensitizer on dentin permeability and tubule occlusion in extracted human premolars. Materials and methods: Hydraulic conductance (HC) measurement (n = 50) and scanning electron microscopy (SEM; n = 64) were performed. Tooth specimens were divided into 6 groups and treated with: G1, distilled water for 30 s; G2, distilled water for 5 min; G3, gel desensitizer for 30 s; G4, gel desensitizer for 5 min; G5, 3% potassium oxalate for 30 s; G6, 3% potassium oxalate for 5 min. HC of dentin were measured before and after 30 s-etching with 35% phosphoric acid, at 0, 30, 60 min after group treatment and after 5-min acid challenge with 6% citric acid. The degree of tubule occlusion and the penetration depth of each agent were also determined. ANOVA and multiple comparison tests were used for data analysis. Results: G3, G4, G5 and G6 significantly decreased in HC after group treatment every observation period, compared to after acid-etching (P < 0.001), and had 100% degree of tubule occlusion with penetration depth about 7.62, 7.94, 8.59 and 8.66 mm, respectively. However, G6 Conclusion: Gel desensitizer treatment, for only 30 s, could reduce dentin permeability and completely occlude the dentinal tubules, even though after acid challenge. However, 5-min treatment with potassium oxalate showed the greatest decrease in dentin permeability. <feminine ordinal indicator> 2025 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons. org/licenses/by-nc-nd/4.0/). showed the greatest reduction in HC (P < 0.05).