RNA-Based Next-Generation Sequencing in Non-Small Cell Lung Cancer patients: data from Campania, Italy

被引：0

作者：

Pisapia, Pasquale ^{[1
]}

Iaccarino, Antonino ^{[1
]}

De Luca, Caterina ^{[1
]}

Pepe, Francesco ^{[1
]}

Russo, Gianluca ^{[1
]}

Nacchio, Mariantonia ^{[1
]}

Ambrosio, Francesca ^{[2
]}

Bianco, Roberto ^{[3
]}

Campione, Severo ^{[4
]}

Caputo, Alessandro ^{[5
]}

Carotenuto, Pietro ^{[6
,7
]}

D'Antonio, Antonio ^{[8
]}

D'Armiento, Maria ^{[1
]}

Damiano, Vincenzo ^{[3
]}

Daniele, Bruno ^{[9
]}

De Chiara, Giovanni ^{[10
]}

De Felice, Marco ^{[11
]}

Della Gravara, Luigi ^{[12
]}

Fabozzi, Teresa ^{[9
]}

Feliciano, Salvatore ^{[13
]}

Gridelli, Cesare ^{[14
]}

Guadagno, Elia ^{[4
]}

Ilardi, Gennaro ^{[15
]}

Leopardo, Davide ^{[16
]}

Libroia, Annamaria ^{[17
]}

Maione, Paolo ^{[14
]}

Morgillo, Floriana ^{[12
]}

Orefice, Jessica ^{[9
]}

Panico, Luigi ^{[18
]}

Rocco, Danilo ^{[19
]}

Servetto, Alberto ^{[3
]}

Varricchio, Silvia ^{[15
]}

Zeppa, Pio ^{[5
]}

Vigliar, Elena ^{[1
]}

Bellevicine, Claudio ^{[1
]}

Troncone, Giancarlo ^{[1
]}

Malapelle, Umberto ^{[1
]}

机构：

[1] Univ Naples Federico II, Dept Publ Hlth, Naples, Italy

[2] Hosp Antonio Cardarelli, Oncol Unit, AORN Cardarelli, Naples, Italy

[3] Univ Naples Federico II, Dept Clin Med & Surg, Naples, Italy

[4] Hosp Antonio Cardarelli, Pathol Unit, AORN Cardarelli, Naples, Italy

[5] Univ Hosp Salerno, Dept Pathol, Salerno, Italy

[6] Univ Naples Federico II, Dept Translat Med Sci, Med Genet, Naples, Italy

[7] Telethon Inst Genet & Med, TIGEM, Naples, Italy

[8] Osped Mare, Pathol Unit, Naples, Italy

[9] Osped Mare, Oncol Unit, Naples, Italy

[10] SG Moscati Hosp, Div Pathol, Avellino, Italy

[11] Osped Ave Gratia Plena, Med Oncol Unit, Caserta, Italy

[12] Univ Campania Luigi Vanvitelli, Dept Precis Med, Naples, Italy

[13] Osped Ave Gratia Plena, Med Oncol Unit, ASL Caserta, Caserta, Italy

[14] SG Moscati Hosp, Div Med Oncol, Avellino, Italy

[15] Univ Naples Federico II, Dept Adv Biomed Sci, Naples, Italy

[16] AORN St Anna & San Sebastiano, Dept Oncol, Caserta, Italy

[17] Andrea Tortora Hosp, Oncol Unit, ASL Salerno, Pagani, Italy

[18] AORN Colli Monaldi, Dept Pathol, Naples, Italy

[19] AORN Colli Monaldi, Dept Pulm Oncol, Naples, Italy

来源：

PATHOLOGICA | 2024年 / 116卷 / 05期

关键词：

NSCLC; NGS; predictive molecular pathology; molecular oncology; RNA-based biomarkers;

D O I：

10.32074/1591-951X-1015

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Objective. ALK, ROS1, NTRK, and RET gene fusions and MET exon 14 skipping alterations represent fundamental predictive biomarkers for advanced non-small cell lung cancer (NSCLC) patients to ensure the best treatment choice. In this scenario, RNA-based NGS approach has emerged as an extremely useful tool for detecting these alterations. In this study, we report our NGS molecular records on ALK, ROS1, NTRK, and RET gene fusions and MET exon 14 skipping alterations detected by using a narrow RNA-based NGS panel, namely SiRe fusion. Methods. We retrospectively reviewed data on 201 advanced stage NSCLC patients who were referred to our laboratory for RNA-based molecular evaluation of ALK, ROS1, RET, NTRK gene rearrangements as well as MET exon 14 skipping. Results. Overall, 23 (11.4%) positive cases were retrieved. Regarding molecular assessment, 11 (5.5%), 2 (1.0%), 9 (4.5%), and 1 (0.5%) out of 201 harbored an ALK, ROS1, RET gene rearrangement, or MET exon 14 skipping, respectively. Conclusions. In this study, we provide real-world experience on RNA-based NGS analysis in patients with advanced stage NSCLC.

引用

页码：310 / 319

页数：10

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