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Ultrasound-assisted deep eutectic solvent extraction of flavonoids from pericarp of cultivated 'Qi-nan' agarwood: identification, optimization, and antioxidant activity
被引:0
|作者:
Huang, Mei
[1
,2
,3
]
Fu, Yunlin
[1
,2
,3
]
Wei, Penglian
[1
,2
,3
]
Li, Yingjian
[1
,2
,3
]
机构:
[1] Guangxi Univ, Coll Forestry, Nanning 530004, Peoples R China
[2] Guangxi Coll & Univ, Key Lab Cultivat & Utilizat Subtrop Forest Plantat, Nanning 530004, Peoples R China
[3] Guangxi Key Lab Forest Ecol & Conservat, Nanning 530004, Peoples R China
基金:
中国国家自然科学基金;
关键词:
'Qi-nan' pericarp;
Agarwood;
Flavonoids;
Identification;
Green extraction;
Deep eutectic solvents;
Antioxidant activity;
PHENOLIC COMPOSITION;
BIOACTIVE COMPOUNDS;
L;
FRUITS;
D O I:
10.1016/j.microc.2025.113303
中图分类号:
O65 [分析化学];
学科分类号:
070302 ;
081704 ;
摘要:
With the expansion of the cultivation area of 'Qi-nan' species, the amount of fruit resources has increased correspondingly. However, each felling for agarwood results in many 'Qi-nan' fruits being discarded, resulting in significant resource waste. To investigate the chemical constituents of 'Qi-nan' fruits and improve the utilization of their resources, this study employed ultrasound-assisted deep eutectic solvent extraction (UA-DES) to extract flavonoids from the 'Qi-nan' pericarp(FQP). First, 13 flavonoids, including quercetin, and kaempferol, were identified from the 'Qi-nan' pericarp using ultra-performance liquid chromatography-mass spectrometry (UPLCMS). Next, the highest yield of FQP reached 12.03 f 0.24 %, significantly higher than that obtained with 50 % ethanol, and enhanced by 1.44 times, using a molar ratio of 1:2 choline chloride/urea(ChCl/urea (1:2)) as the optimal solvent, a 40 % water content, a 30 mL/g liquid-to-solid ratio, 40 min ultrasonication time. Additionally, scanning electron microscopy (SEM) indicated that the treatment with ChCl/urea (1:2) caused greater destruction of the sample compared to traditional solvent ethanol. The antioxidant activity indicated that the IC50 values of ChCl/urea (1:2) against DPPH and ABTS+ radicals were 30.66 f 0.36 and 47.13 f 0.79 mu g/mL, respectively, while those of ethanol extracts were 37.23 f 0.13 and 48.08 f 0.15 mu g/mL. The FRAP slopes for ChCl/urea (1:2) extract and ethanol extract were 0.2216 and 0.1835, respectively. This suggests that UA-DES is a more efficient and active method for extracting FQP than conventional solvent extraction. This study provides data support to the development and use of the 'Qi-nan' pericarp, along with a sustainable efficient method for the extraction of FQP.
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页数:11
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