Counter-diffusion studies of human transthyretin: the growth of high-quality crystals for X-ray and neutron crystallography

被引：0

作者：

De'Ath, Clare ^{[1
,2
,3
,9
]}

Oliva, Mizar F. ^{[1
,3
,10
]}

Moulin, Martine ^{[1
,3
]}

Blakeley, Matthew P. ^{[4
]}

Haertlein, Michael ^{[1
,3
]}

Mitchell, Edward P. ^{[2
,3
]}

Gavira, Jose A. ^{[5
]}

Bowler, Matthew W. ^{[6
]}

Forsyth, V. Trevor ^{[7
,8
]}

机构：

[1] Inst Laue Langevin, Life Sci Grp, 71 Ave Martyrs, F-38042 Grenoble, France

[2] European Synchrotron Radiat Facil, 71 Ave Martyrs, F-38042 Grenoble, France

[3] Partnership Struct Biol, 71 Ave Martyrs, F-38042 Grenoble, France

[4] Inst Laue Langevin, Large Scale Struct Grp, 71 Ave Martyrs, F-38042 Grenoble, France

[5] CSIC, Inst Andaluz Ciencias Tierra, Lab Estudios Cristalog, Ave Las Palmeras 4, Granada 18100, Spain

[6] European Mol Biol Lab, 71 Ave Martyrs, F-38042 Grenoble, France

[7] Lund Univ, Fac Med, SE-22184 Lund, Sweden

[8] LINXS Inst Adv Neutron & Xray Sci, Scheelevagen 19, SE-22370 Lund, Sweden

[9] Univ Oxford, Sir William Dunn Sch Pathol, S Parks Rd, Oxford OX1 3RE, England

[10] Katholieke Univ Leuven, Dept Microbiol Immunol & Transplantat, Rega Inst Med Res, Leuven, Belgium

来源：

JOURNAL OF APPLIED CRYSTALLOGRAPHY | 2025年 / 58卷

基金：

瑞典研究理事会;

关键词：

counter-diffusion crystallization; X-ray diffraction; neutron macromolecular crystallography; perdeuteration; large-volume crystals; transthyretin; crystallogenesis; macromolecular deuteration; amyloidogenesis; FULLY-AUTOMATIC CHARACTERIZATION; PROTEIN CRYSTALS; DATA-COLLECTION; MACROMOLECULAR CRYSTALLOGRAPHY; CAPILLARY COUNTERDIFFUSION; RESOLUTION NEUTRON; SAMPLE-EVALUATION; ROOM-TEMPERATURE; CRYSTALLIZATION; DIFFRACTION;

D O I：

10.1107/S1600576724011191

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A crystallogenesis study of human transthyretin using the counter-diffusion method is described as an alternative to conventional convective vapour diffusion and batch approaches for protein crystallization. The X-ray diffraction results show systematic trends that exhibit unique patterns of crystallization and high crystal quality as well as a remarkable degree of coherence within extended crystal rods that wholly fill the capillaries used. Preliminary neutron diffraction data have been recorded from a number of these samples, validating the feasibility of this methodology for neutron crystallography.

引用

页码：107 / 118

页数：12

共 75 条

[11] Diffraction cartography: applying microbeams to macromolecular crystallography sample evaluation and data collection [J].

Bowler, Matthew W. ;

Guijarro, Matias ;

Petitdemange, Sebastien ;

Baker, Isabel ;

Svensson, Olof ;

Burghammer, Manfred ;

Mueller-Dieckmann, Christoph ;

Gordon, Elspeth J. ;

Flot, David ;

McSweeney, Sean M. ;

Leonard, Gordon A. .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2010, 66 :855-864

[12] Measurement of the intrinsic variability within protein crystals: implications for sample-evaluation and data-collection strategies [J].

Bowler, Michael G. ;

Bowler, Matthew W. .

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2014, 70 :127-132

[13] Near-Atomic Resolution Neutron Crystallography on Perdeuterated Pyrococcus furiosus Rubredoxin: Implication of Hydronium Ions and Protonation State Equilibria in Redox Changes [J].

Cuypers, M. G. ;

Mason, S. A. ;

Blakeley, M. P. ;

Mitchell, E. P. ;

Haertlein, M. ;

Forsyth, V. Trevor .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2013, 52 (03) :1022-1025

[14] Direct visualization of critical hydrogen atoms in a pyridoxal 5′-phosphate enzyme [J].

Dajnowicz, Steven ;

Johnston, Ryne C. ;

Parks, Jerry M. ;

Blakeley, Matthew P. ;

Keen, David A. ;

Weiss, Kevin L. ;

Gerlits, Oksana ;

Kovalevsky, Andrey ;

Mueser, Timothy C. .

NATURE COMMUNICATIONS, 2017, 8

[15] Neutron diffraction from a microgravity-grown crystal reveals the active site hydrogens of the internal aldimine form of tryptophan synthase [J].

Drago, Victoria N. ;

Devos, Juliette M. ;

Blakeley, Matthew P. ;

Forsyth, V. Trevor ;

Parks, Jerry M. ;

Kovalevsky, Andrey ;

Mueser, Timothy C. .

CELL REPORTS PHYSICAL SCIENCE, 2024, 5 (02)

[16] An NMIDLINE HORIZONTAL ELLIPSISHMIDLINE HORIZONTAL ELLIPSISN low-barrier hydrogen bond preorganizes the catalytic site of aspartate aminotransferase to facilitate the second half-reaction [J].

Drago, Victoria N. ;

Dajnowicz, Steven ;

Parks, Jerry M. ;

Blakeley, Matthew P. ;

Keen, David A. ;

Coquelle, Nicolas ;

Weiss, Kevin L. ;

Gerlits, Oksana ;

Kovalevsky, Andrey ;

Mueser, Timothy C. .

CHEMICAL SCIENCE, 2022, 13 (34) :10057-10065

[17] Microgravity crystallization of perdeuterated tryptophan synthase for neutron diffraction [J].

Drago, Victoria N. ;

Devos, Juliette M. ;

Blakeley, Matthew P. ;

Forsyth, V. Trevor ;

Kovalevsky, Andrey Y. ;

Schall, Constance A. ;

Mueser, Timothy C. .

NPJ MICROGRAVITY, 2022, 8 (01)

[18] Revealing protonation states and tracking substrate in serine hydroxymethyltransferase with room-temperature X-ray and neutron crystallography [J].

Drago, Victoria N. N. ;

Campos, Claudia ;

Hooper, Mattea ;

Collins, Aliyah ;

Gerlits, Oksana ;

Weiss, Kevin L. L. ;

Blakeley, Matthew P. P. ;

Phillips, Robert S. S. ;

Kovalevsky, Andrey .

COMMUNICATIONS CHEMISTRY, 2023, 6 (01)

[19] How good are my data and what is the resolution? [J].

Evans, Philip R. ;

Murshudov, Garib N. .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2013, 69 :1204-1214

[20] TIM crystals grown by capillary counterdiffusion: Statistical evidence of quality improvement in microgravity [J].

Evrard, Christine ;

Maes, Dorninique ;

Zegers, Ingrid ;

Declercq, Jean-Paul ;

Vanhee, Celine ;

Martial, Joseph ;

Wyns, Lode ;

Van De Weerdt, Ceile .

CRYSTAL GROWTH & DESIGN, 2007, 7 (11) :2161-2166

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