Counter-diffusion studies of human transthyretin: the growth of high-quality crystals for X-ray and neutron crystallography

被引：0

作者：

De'Ath, Clare ^{[1
,2
,3
,9
]}

Oliva, Mizar F. ^{[1
,3
,10
]}

Moulin, Martine ^{[1
,3
]}

Blakeley, Matthew P. ^{[4
]}

Haertlein, Michael ^{[1
,3
]}

Mitchell, Edward P. ^{[2
,3
]}

Gavira, Jose A. ^{[5
]}

Bowler, Matthew W. ^{[6
]}

Forsyth, V. Trevor ^{[7
,8
]}

机构：

[1] Inst Laue Langevin, Life Sci Grp, 71 Ave Martyrs, F-38042 Grenoble, France

[2] European Synchrotron Radiat Facil, 71 Ave Martyrs, F-38042 Grenoble, France

[3] Partnership Struct Biol, 71 Ave Martyrs, F-38042 Grenoble, France

[4] Inst Laue Langevin, Large Scale Struct Grp, 71 Ave Martyrs, F-38042 Grenoble, France

[5] CSIC, Inst Andaluz Ciencias Tierra, Lab Estudios Cristalog, Ave Las Palmeras 4, Granada 18100, Spain

[6] European Mol Biol Lab, 71 Ave Martyrs, F-38042 Grenoble, France

[7] Lund Univ, Fac Med, SE-22184 Lund, Sweden

[8] LINXS Inst Adv Neutron & Xray Sci, Scheelevagen 19, SE-22370 Lund, Sweden

[9] Univ Oxford, Sir William Dunn Sch Pathol, S Parks Rd, Oxford OX1 3RE, England

[10] Katholieke Univ Leuven, Dept Microbiol Immunol & Transplantat, Rega Inst Med Res, Leuven, Belgium

来源：

JOURNAL OF APPLIED CRYSTALLOGRAPHY | 2025年 / 58卷

基金：

瑞典研究理事会;

关键词：

counter-diffusion crystallization; X-ray diffraction; neutron macromolecular crystallography; perdeuteration; large-volume crystals; transthyretin; crystallogenesis; macromolecular deuteration; amyloidogenesis; FULLY-AUTOMATIC CHARACTERIZATION; PROTEIN CRYSTALS; DATA-COLLECTION; MACROMOLECULAR CRYSTALLOGRAPHY; CAPILLARY COUNTERDIFFUSION; RESOLUTION NEUTRON; SAMPLE-EVALUATION; ROOM-TEMPERATURE; CRYSTALLIZATION; DIFFRACTION;

D O I：

10.1107/S1600576724011191

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A crystallogenesis study of human transthyretin using the counter-diffusion method is described as an alternative to conventional convective vapour diffusion and batch approaches for protein crystallization. The X-ray diffraction results show systematic trends that exhibit unique patterns of crystallization and high crystal quality as well as a remarkable degree of coherence within extended crystal rods that wholly fill the capillaries used. Preliminary neutron diffraction data have been recorded from a number of these samples, validating the feasibility of this methodology for neutron crystallography.

引用

页码：107 / 118

页数：12

共 75 条

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