Advanced diffusion-weighted imaging-derived quantitative parameters as biomarkers of fibrosarcoma-cell proliferation in nude mice: A study based on precise imaging-pathology correlation

被引：0
作者：
Huang, Jie [1 ,2 ]
Duan, Zhiqing [2 ]
Cheng, Yu [2 ]
Tao, Juan [3 ]
Dai, Siyu [4 ,5 ]
Zhou, Jianwen
Wang, Shaowu [2 ]
机构：
[1] Dalian Med Univ, Dalian, Peoples R China
[2] Dalian Med Univ, Hosp 2, Dept Radiol, 467 Zhongshan Rd, Dalian, Peoples R China
[3] Dalian Med Univ, Hosp 2, Dept Pathol, Dalian, Peoples R China
[4] Hangzhou Normal Univ, Sch Clin Med, Hangzhou, Peoples R China
[5] Chinese Univ Hong Kong, Fac Med, Hong Kong, Peoples R China
来源：
MAGNETIC RESONANCE IMAGING | 2025年 / 118卷
基金：
中国国家自然科学基金;
关键词：
Fibrosarcoma; Nude mouse; Immunohistochemistry; Aquaporins; Ki-67; Cell density; Diffusion kurtosis imaging; Intravoxel incoherent motion; SOFT-TISSUE SARCOMAS; TUMOR-GROWTH; AQUAPORIN-1; EXPRESSION; ANGIOGENESIS; MIGRATION;
D O I：
10.1016/j.mri.2025.110345
中图分类号：
R8 [特种医学]; R445 [影像诊断学];
学科分类号：
1002 ; 100207 ; 1009 ;
摘要：
Purpose: To determine whether quantitative parameters derived using diffusion kurtosis imaging (DKI) and intravoxel incoherent motion (IVIM) imaging reflect pathological changes in fibrosarcoma. Methods: Thirty nude mouse models of fibrosarcoma underwent T1/T2-weighted imaging, DKI, and IVIM imaging on a 3.0-T scanner. Immunohistochemistry was utilized for the hematoxylin and eosin, aquaporin 1 (AQP1), aquaporin 4 (AQP4), and Ki-67 staining of fibrosarcoma tissue, and AQP1 and AQP4 staining of normal muscle tissue (NMT). The independent-sample t-test was used to compare AQP1 and AQP4 expression in fibrosarcoma and NMT. Pearson and Spearman correlation analyses were conducted to evaluate the correlation between imaging parameters and pathological indicators. Multiple linear regression analysis was employed to identify the pathological indicators independently associated with quantitative DKI and IVIM parameters. Results: Apparent diffusion coefficient (ADC), D, f, and mean kurtosis (MK) indicated cell density and Ki-67 and AQP1 expression intensity. D values reflected AQP4 expression intensity, while MD reflected cell density and AQP1 expression intensity. Cell density (CD) independently influenced ADC and f values, while CD and AQP1 independently influenced D values. Conclusion: CD and Ki-67 independently influenced MK. DKI- and IVIM imaging-derived ADC, D, f, MD, and MK were correlated with AQP1, AQP4, Ki-67, and CD in nude mice with fibrosarcoma.
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页数：9
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