Divergent response of Chernozem organic matter towards short-term water stress in Poa pratensis L. rhizosphere and bulk soil in pot experiments: A

Understanding and controlling rhizospheric processes under abiotic stress is one of the key challenges in addressing food security amid the climate crisis. In this work, the impact of short-term drought and overwatering on soil organic matter (SOM) of Haplic Chernozem in the rhizosphere of Poa pratensis L. and in bulk soil was investigated. The vegetation experiment was conducted in a climatic chamber at soil moisture levels of 35, 80, and 200 % of the field capacity. UV-Vis and spectrofluorometry were used to describe the water-extractable organic matter (WEOM) characteristics and fluorofores signature, and diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) to describe functional group composition of SOM. Composition and properties of SOM and WEOM of Chernozem significantly change after exposure to short-term water stress. Drought does not affect the composition of rhizosphere SOM except increasing the proportion of polysaccharides, but leads to the decrease in aromaticity and increase in molecular weight of humic-like components of rhizosphere WEOM. These findings reflect Poa adaptation to water deficiency and microbial activity suppression which results in accumulation of SOM intermediate decomposition products. On the contrary, bulk WEOM wasn't affected by drought but SOM became enriched with aromatic and oxidised components. Overwatering leads to equalisation of bulk and rhizospheric SOM composition due to a decrease in the proportion of aromatic and carboxylic components of bulk SOM and the accumulation of microbial products in both bulk and rhizospheric SOM. In general, rhizospheric WEOM undergoes relatively significant changes relative to the optimum water regime under moisture deficit, and bulk WEOM - under overwatering. The findings illustrate the involvement of the both WEOM and SOM in maintaining resilience of the soil-plant system as well as the difference in watering conditions impact on SOM in rhizosphere and bulk soil. SOM spectral data can be used for assessing the state of soil systems, such as changes in microbial activity and adaptation of the soil-plant system to abiotic stress. Our findings also illustrate the differences in the organic matter transformation of the Poa pratensis rhizosphere and the bulk Chernozem depending on environmental factors.