Study on Single Nucleotide Polymorphism of LAP3 Gene and Its Correlation with Dairy Quality Traits of Gannan Yak

被引：4

作者：

Wang, Tong ^{[1
,2
]}

Ma, Xiaoming ^{[1
,2
]}

Feng, Fen ^{[1
,2
]}

Zheng, Fei ^{[3
]}

Zheng, Qingbo ^{[1
,2
]}

Zhang, Juanxiang ^{[1
,2
]}

Zhang, Minghao ^{[1
,2
]}

Ma, Chaofan ^{[1
,2
,3
]}

Deng, Jingying ^{[1
,2
]}

Guo, Xian ^{[1
,2
]}

Chu, Min ^{[1
,2
]}

La, Yongfu ^{[1
,2
]}

Bao, Pengjia ^{[1
,2
]}

Pan, Heping ^{[3
]}

Liang, Chunnian ^{[2
]}

Yan, Ping ^{[1
,4
]}

机构：

[1] Chinese Acad Agr Sci, Lanzhou Inst Husb & Pharmaceut Sci, Key Lab Yak Breeding Engn Gansu Prov, Lanzhou 730000, Peoples R China

[2] Minist Agr & Rural Affairs, Key Lab Anim Genet & Breeding Tibetan Plateau, Lanzhou 730000, Peoples R China

[3] Northwest Minzu Univ, Life Sci & Engn Coll, Lanzhou 730124, Peoples R China

[4] Chinese Acad Agr Sci, Inst Western Agr, Changji 831100, Peoples R China

来源：

FOODS | 2024年 / 13卷 / 18期

基金：

中国国家自然科学基金;

关键词：

Gannan yak; LAP3; gene; SNPs; molecular marker; MILK-PRODUCTION TRAITS; LEUCINE AMINOPEPTIDASE; IDENTIFICATION; ALIGNMENT; CASEIN; FAT; QTL;

D O I：

10.3390/foods13182953

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

This study explored the polymorphism of the leucine aminopeptidase (LAP3) gene and its relationship with milk quality characteristics in Gannan yak. A cohort of 162 Gannan yak was genotyped utilizing the Illumina Yak cGPS 7K BeadChip, and the identified single nucleotide polymorphisms (SNPs) were evaluated for their association with milk protein, casein, lactose, and fat concentrations. The results showed that four SNPs (g.4494G > A, g.5919A > G, g.8033G > C, and g.15,615A > G) in the LAP3 gene exhibited polymorphism with information content values of 0.267, 0.267, 0.293, and 0.114, respectively. All four SNPs were in Hardy-Weinberg equilibrium (p > 0.05). The g.4494G > A and g.5919A > G SNPs were significantly associated with protein content (p < 0.05), with homozygous genotypes showing significantly higher protein content than heterozygous genotypes (p < 0.05). The g.8033G > C SNP was significantly associated with casein content, protein content, non-fat solids, and acidity (p < 0.05), with the CC genotype having significantly higher casein, protein, and non-fat solids content than the GG and GC genotypes (p < 0.05). The g.15,615A > G SNP was significantly associated with average fat globule diameter (p < 0.05). In general, the mutations within the LAP3 gene demonstrated a positive impact on milk quality traits in Gannan yak, with mutated genotypes correlating with enhanced milk quality. These results indicate that the LAP3 gene could be a significant or candidate gene affecting milk quality traits in Gannan yak and offer potential genetic markers for molecular breeding programs in this species.

引用

页数：11

共 48 条

[1] Genome-Wide SNP Analysis for Milk Performance Traits in Indigenous Sheep: A Case Study in the Egyptian Barki Sheep [J].