Neuroprotective Effects of Ethanol Extract Polyscias guilfoylei (EEPG) Against Glutamate Induced Neurotoxicity in HT22 Cells

被引：2

作者：

Nguyen, Qui Ngoc Sang ^{[1
,2
,3
,4
]}

Yoo, Ki-Yeon ^{[2
,3
]}

Pham, Thi Thu Trang ^{[1
,5
]}

Selvaraj, Baskar ^{[1
]}

Vu, Huong Thuy ^{[6
,7
]}

Le, Tam Thi ^{[1
]}

Lee, Heesu ^{[2
,3
]}

Tran, Quang Luc ^{[6
]}

Thuong, Phuong Thien ^{[8
]}

Pae, Ae Nim ^{[9
,10
]}

Jung, Sang Hoon ^{[1
,5
]}

Lee, Jae Wook ^{[1
,5
]}

机构：

[1] Korea Inst Sci & Technol, Inst Nat Prod, Nat Prod Res Ctr, Kangnung 25451, South Korea

[2] Gangneung Wonju Natl Univ, Coll Dent, Dept Anat, 7 Jukheon Gil, Kangnung 25457, South Korea

[3] Gangneung Wonju Natl Univ, Res Inst Dent Engn, 7 Jukheon Gil, Kangnung 25457, South Korea

[4] Vietnamese Acad Sci & Technol, Inst Nat Prod Chem, 1H Bldg,18 Hoang Quoc Viet St, Hanoi 100000, Vietnam

[5] Univ Sci & Technol UST, KIST Sch, Nat Prod Appl Sci, Kangnung 25451, South Korea

[6] Traphaco Join Stock Co, 75 P Yen Ninh, Hanoi 1000000, Vietnam

[7] Hanoi Univ Pharm, Fac Herbal Med, Tradit Pharm, 13-15 Le Thanh Tong, Hanoi 100000, Vietnam

[8] Vietnam Korea Inst Sci & Technol, Div Biotechnol, Hoa Lac High Tech Pk,Km29 Thang Long Blvd, Hanoi 100000, Vietnam

[9] Korea Univ Sci & Technol UST, KIST Sch, Div Biomed Sci & Technol, Seoul 02792, South Korea

[10] Korea Inst Sci & Technol, Brain Res Inst, Ctr Brain Disorders, Seoul 02792, South Korea

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2024年 / 25卷 / 22期

基金：

新加坡国家研究基金会;

关键词：

Polyscias guilfoylei; HT22; cells; neuroprotection; ischemic brain injury; OXIDATIVE STRESS; DEATH; DISEASE; AIF;

D O I：

10.3390/ijms252212153

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oxidative stress induced by glutamate is a significant contributor to neuronal cell damage and can lead to neurodegenerative diseases such as Alzheimer's, Huntington's, and ischemic brain injury. At the cellular level, oxidative stress increases Ca2+ ion influx and reactive oxygen species (ROS), which activate the MAPK signaling pathway. Additionally, the generation of ROS causes mitochondrial dysfunction, triggering apoptosis by promoting the translocation of AIF to the nucleus from the mitochondria. The neuroprotective potential of Polyscias guilfoylei has not yet been reported. Therefore, in this study, the ethanol extract of Polyscias guilfoylei (EEPG) was examined for its protective effect against oxidative cell damage caused by glutamate in neuronal cells. EEPG treatment increased the viability of HT22 cells exposed to high concentrations of glutamate. Cellular Ca2+ ion influx and ROS generation decreased with EEPG treatment in glutamate-treated HT22 cells. EEPG treatment inhibited MAPK activation and AIF nuclear translocation. In an in vivo study, EEPG attenuated brain cell death in an ischemic brain injury rat model. This study demonstrates the potential therapeutic effects of Polyscias guilfoylei in the treatment of ischemic brain injury.

引用

页数：14

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