Detecting Arboviruses Through Screening Asymptomatic Blood Donors in Rio de Janeiro/Brazil During a Dengue Outbreak

被引：0

作者：

Ribeiro, Marisa de Oliveira ^{[1
]}

Arruda, Monica Barcellos ^{[1
]}

Calazans, Alexandre Rodrigues ^{[1
]}

Frederico, Alexandre Vicente ^{[1
]}

Brito, Anielly Ferreira ^{[1
]}

Barreto, Beatriz Vasconcello de Souza ^{[1
]}

Brandao, elida Millena de Vasconcelos ^{[1
]}

Athayde, Hamilton ^{[1
]}

Nascimento, Katia Cristina Silva ^{[1
]}

Souza, Luiz Paulo de Brito Oliveira ^{[1
]}

Cardoso, Pedro Henrique ^{[1
]}

Guimaraes, Priscilla Lopes da Silva ^{[1
]}

da Costa, Vanessa Duarte ^{[1
]}

Silva, Carlos Alexandre da Costa ^{[2
]}

Soares, Alexandra Martins ^{[2
]}

Iole, Josiane ^{[2
]}

Louzada, Guilherme ^{[2
]}

Filho, Luiz Amorim ^{[2
]}

Alvarez, Patricia ^{[1
]}

机构：

[1] Fundacao Oswaldo Cruz Fiocruz, Lab Kits Mol LAMOL, Inst Tecnol Imunobiol Bio Manguinhos, BR-21040360 Rio De Janeiro, Brazil

[2] Inst Estadual Hematol, HEMORIO, BR-20211030 Rio De Janeiro, Brazil

来源：

VIRUSES-BASEL | 2025年 / 17卷 / 02期

关键词：

arboviruses; blood donors; hemocenter; ZIKA VIRUS; CHIKUNGUNYA; INFECTION; DISEASES; TRANSFUSION; STATES;

D O I：

10.3390/v17020224

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Arthropod-borne viruses (arboviruses) dengue (DENV), chikungunya (CHIK), and Zika (ZIKV) have been responsible for a high number of outbreaks worldwide. However, their screening in blood donors is not mandatory, and asymptomatic cases might act as an important cause of virus transmission via transfusion. A study was conducted to assess the presence of DENV (serotypes 1-4), ZIKV, and CHIKV in pooled samples (pool size: six) from asymptomatic blood donors. A total of 9463 plasma pools, corresponding to 56,778 blood donations from asymptomatic blood donors who attended donor sessions at HEMORIO and other blood centers in Rio de Janeiro and Esp & iacute;rito Santo, was submitted to automated nucleic-acid extraction and PCR amplification using ZC D-Tipagem molecular assay (Bio-Manguinhos). In general, a pool prevalence of 1% (95/9463) and a donor prevalence of 0.17% (95/56,778) were observed. January and February 2024 had a total of 62 positive pools out of 95 (65.3%). Targets DENV-1 and -2 had a higher prevalence in the studied months-early summer-with 24 and 28 positive pools, respectively. ZC D-Tipagem molecular assay was able to detect the best-known arboviruses circulating in asymptomatic blood donors; this study suggested that ZIKV, CHIK, and DENV are circulating in asymptomatic blood donors before blood donations and can be transmitted to blood transfusion recipients.

引用

页数：7

共 26 条

[1]

Mayer S.V., Tesh R.B., Vasilakis N., The emergence of arthropod-borne viral diseases: A global prospective on dengue, chikungunya and zika fevers, Acta Trop, 166, pp. 155-163, (2017)

[2]

Sharma R., Costa Santos L., da Silva R.A., Goncalves C.V., de Melo Calado S., Santos D.P., de Andrade de Melo J.P., de Cassia Pontello Rampazzo R., Requiao L., Krieger M.A., Et al., Surveillance of donated blood during the 2016 arbovirus outbreak in Brazil, J. Med. Virol, 90, pp. 1406-1410, (2018)

[3]

Vector-Borne Diseases, (2020)

[4]

Gubler D.J., The global emergence/resurgence of arboviral diseases as public health problems, Arch. Med. Res, 33, pp. 330-342, (2002)

[5]

Weaver S.C., Urbanization and geographic expansion of zoonotic arboviral diseases: Mechanisms and potential strategies for prevention, Trends Microbiol, 21, pp. 360-363, (2013)

[6]

Murugesan A., Manoharan M., Dengue virus, Emerging and Reemerging Viral Pathogens, pp. 281-359, (2020)

[7]

Atualização de Casos de Arboviroses, (2024)

[8]

Madariaga M., Ticona E., Resurrecion C., Chikungunya: Bending over the Americas and the rest of the world, Braz. J. Infect. Dis, 20, pp. 91-98, (2016)

[9]

Dick G.W., Kitchen S.F., Haddow A.J., Zika virus I. Isolations and serological specificity, Trans. R. Soc. Trop. Med. Hyg, 46, pp. 509-520, (1952)

[10]

Slavov S.N., Otaguiri K.K., Kashima S., Covas D.T., Overview of Zika virus (ZIKV) infection in regard to the Brazilian epidemic, Braz. J. Med. Biol. Res, 49, (2016)

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