Antiparasitic activity of Colombian Amazon palm extracts against Giardia lamblia trophozoites: insights into cellular death mechanisms

被引：0

作者：

Garcia-Bustos, Juan Javier ^{[1
,2
]}

Pizarro, Gabriel Luna ^{[3
]}

Patolsky, Rocio G. ^{[3
]}

Joray, Mariana Belen ^{[4
]}

Villalba-Vizcaino, Vivian ^{[2
]}

Galeano, Paula ^{[5
]}

Espitia-Almeida, Fabian ^{[6
,7
]}

Munera, Marco Correa ^{[5
]}

Ozturk, Mehmet ^{[8
,9
]}

Ropolo, Andrea S. ^{[3
]}

Feliziani, Constanza ^{[3
]}

Touz, Maria Carolina ^{[3
]}

Laiolo, Jeronimo ^{[3
,10
]}

机构：

[1] Univ Amazonia, Programa Med Vet & Zootecnia, Florencia, Caqueta, Colombia

[2] Univ Magdalena, Fac Ciencias Salud, Doctorado Med Trop SUE Caribe, Grp Invest Inmunol & Patol GIPAT, Santa Marta, Colombia

[3] Univ Nacl Cordoba, Consejo Nacl Invest Cient & Tecn INIMEC CONICET, Inst Invest Med Mercedes & Martin Ferreyra, Cordoba, Argentina

[4] Univ Catolica Cordoba, Ctr Invest & Desarrollo Inmunol & Enfermedades Inf, Consejo Nacl Invest Cient & Tecn CIDIE CONICET UCC, Cordoba, Argentina

[5] Univ Amazonia, Fac Ciencias Basicas, Florencia, Caqueta, Colombia

[6] Univ Simon Bolivar, Fac Ciencias Basicas & Biomed, Ctr Invest Ciencias Vida, Barranquilla, Colombia

[7] Univ Atlantico, Fac Ciencias Basicas, Programa Biol, Puerto Colombia, Colombia

[8] Mugla Sitki Kocman Univ, Fac Sci, Dept Chem, Mugla, Turkiye

[9] Al Farabi Kazakh Natl Univ, Fac Chem & Chem Technol, Alma Ata, Kazakhstan

[10] Univ Catolica Cordoba, Fac Ciencias Salud, Cordoba, Argentina

来源：

FRONTIERS IN MICROBIOLOGY | 2025年 / 16卷

关键词：

Colombian Amazon; Giardia lamblia; giardicidal activity; medicinal plants; plant extracts; RECEPTOR-MEDIATED ENDOCYTOSIS; OXIDATIVE STRESS; LEAF EXTRACTS; METRONIDAZOLE; DUODENALIS; TRAFFICKING; ASSEMBLAGE; RESISTANCE; GROWTH; CYCLE;

D O I：

10.3389/fmicb.2025.1523880

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Introduction Colombian plants have a long history of use in traditional medicine and ethnopharmacology, particularly for treating stomach pain, digestive issues, diarrhea, and other gastrointestinal disorders. Recent studies have renewed interest in their potential therapeutic properties.Methods This study evaluated the giardicidal activity of 15 crude plant extracts native to the Colombian Amazon against Giardia lamblia (genotype A, strain WB/1267). The MTT colorimetric assay was used to determine the effectiveness of these extracts at a concentration of 500 mu g/mL. Extracts showing significant activity were further analyzed to determine their half-maximal inhibitory concentration (IC50). The cell death mechanisms of Attalea butyracea were studied using flow cytometry, confocal microscopy, and transmission electron microscopy (TEM).Results Among the tested extracts, the Attalea butyracea fruit extract (P-2) exhibited the highest activity against WB/1267 (IC50 = 62.10 +/- 6.57 mu g/mL) and demonstrated giardicidal activity against GS/M (IC50 = 100.90 +/- 3.40 mu g/mL, genotype B) human infecting strains. These results prompted a detailed investigation into its mechanism of action using the WB/1267 strain as a model. At its IC50 concentration, P-2 primarily exerted its antiproliferative effect by induction of early apoptosis. A notable increase in late apoptosis and necrosis was observed at 2xIC50. Immunofluorescence assay (IFA) and confocal microscopy revealed chromatin condensation in treated trophozoites, while flow cytometry indicated G1/S cell cycle arrest. Furthermore, exposure to P-2 led to oxidative stress, evidenced by a significant increase in reactive oxygen species (ROS). The extract's ability to disrupt various structural components of the parasite was confirmed through IFA and transmission electron microscopy. Interestingly, the P-2 extract effectively synergized with the first-line drug metronidazole against Giardia WB/1267 trophozoites.Discussion These findings underscore the therapeutic potential of Colombian plant extracts in treating giardiasis, particularly highlighting the novel giardicidal activity of Attalea butyracea fruit extract and its promise for further therapeutic development.

引用

页数：15