Oligochitosan Mitigates Vibrio harveyi Infection in Hybrid Groupers (Epinephelus lanceolatus ♂ x Epinephelus fuscoguttatus ♀) by Modulating Immune Responses and Disease-Related Pathways

Vibrio harveyi is an important pathogen causing enteritis in hybrid groupers. However, its effects on the intestinal microbiota and the regulatory effects of oligochitosan on Vibrio-infected groupers are unclear. In this study, we investigated the effects of infection with V. harveyi and oligochitosan administration on the growth performance, immune responses, and intestinal transcriptome profiles of hybrid groupers during a 60-day rearing period. Growth parameters were reduced in Vibrio-infected groupers, while oligochitosan improved the body weight. Biochemical analysis showed reduced antioxidant enzyme activity and increased lipid peroxidation in Vibrio-infected groupers, which were consequently improved by oligochitosan. Transcriptome analysis was used to identify differentially expressed genes in the disease-related and immune system pathways that significantly accumulated in the treatment groups. An evaluation of the immune response showed a significant down-regulation of proinflammatory cytokines, antioxidant genes, and tight-junction proteins in Vibrio-infected groupers, which was partially restored by oligochitosan. Additionally, intestinal apoptosis, indicated by TUNEL signaling, was observed to significantly increase in Vibrio-infected groupers and was subsequently alleviated by oligochitosan supplementation. These results demonstrate the detrimental effects of V. harveyi infection on the growth and immune function of hybrid groupers and the potential of oligochitosan to attenuate these effects.