De Novo Long-Read Genome Assembly and Annotation of the Mosquito Gut-Dwelling Fungus, Smittium minutisporum

被引:1
作者
Prakash, Anusha [1 ]
Wang, Yan [1 ,2 ]
机构
[1] Univ Toronto Scarborough, Dept Biol Sci, Toronto, ON M1C 1A4, Canada
[2] Univ Toronto, Dept Ecol & Evolutionary Biol, Toronto, ON M5S 3B2, Canada
来源
GENOME BIOLOGY AND EVOLUTION | 2024年 / 16卷 / 12期
基金
加拿大自然科学与工程研究理事会;
关键词
Culicidae; endosymbionts; evolution; fungi; insect; PacBio HiFi; SEQUENCE; GENE;
D O I
10.1093/gbe/evae259
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Mosquito guts host a variety of microbes, yet fungi are often overlooked. Smittium (Harpellales, Zoopagomycota) comprises numerous species that are obligate symbionts residing in the hindgut of mosquito larvae. Despite their association with pathogen-bearing vectors, these fungal symbionts remain understudied, largely due to the lack of high-quality genome resources. This limitation has impeded a deeper understanding of their genome biology and adaptive strategies in relation to their mosquito hosts, which may hold significant epidemiological implications. To address this gap, we generated the first reference-quality genome assembly for this group of fungi, using PacBio HiFi long-reads for an axenic culture of Smittium minutisporum, originally isolated from the eastern treehole mosquito, Aedes triseriatus. The genome assembly consists of 53 contigs, spanning a total length of 32.5 Mb, and is predicted to encode 8,254 protein-coding genes, with repetitive regions constituting 25.22% of the genome. Notably, despite being highly contiguous and gap free, the Benchmarking Universal Single-Copy Ortholog analysis suggests a completeness score of 71.8%, implying unusual genome features, possibly shaped by adaptation and specialization within the mosquito gut. This high-quality genome resource will be invaluable for advancing our understanding of mosquito gut-dwelling fungi, their natural history, and their cryptic symbiosis with insect hosts.
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页数:7
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