Anti-staphylococcal, antibiofilm and trypanocidal activities of CrataBL encapsulated into liposomes: Lectin with potential against infectious diseases

被引：3

作者：

Campos, Luis Andre de Almeida ^{[1
]}

da Costa Jr, Sergio Dias ^{[1
]}

Santos, Joao Victor de Oliveira ^{[1
]}

de Souza, Zion Nascimento ^{[1
]}

da Silva, Carlos Eduardo Sales ^{[2
]}

Cristovao-Silva, Ana Catarina ^{[3
]}

Brelaz-de-Castro, Maria Carolina Accioly ^{[3
,4
]}

Pereira, Valeria Rego Alves ^{[3
]}

Paivab, Patricia Maria Guedes ^{[2
]}

Correiab, Maria Tereza dos Santos ^{[2
]}

Santos-Magalhaes, Nereide Stela ^{[1
]}

Cavalcanti, Isabella Macario Ferro ^{[1
,4
]}

机构：

[1] Fed Univ Pernambuco UFPE, Keizo Asami Inst ILIKA, Av Prof Moraes Rego,1235,Cidade Univ, BR-50670901 Recife, PE, Brazil

[2] Fed Univ Pernambuco UFPE, Biochem Dept, Recife, PE, Brazil

[3] Oswaldo Cruz Pernambuco Fdn Fiocruz PE, Immunogenet Lab, Recife, PE, Brazil

[4] Fed Univ Pernambuco UFPE, Acad Ctr Vitoria, Vitoria De Santo Antao, PE, Brazil

来源：

MICROBIAL PATHOGENESIS | 2024年 / 196卷

关键词：

Lectin; Nanocarriers; Antimicrobial activity; Antiparasitic activity; Immunomodulation; TRYPANOSOMA-CRUZI; SUBINHIBITORY CONCENTRATIONS; IN-VITRO; DERIVATIVES; ANTITUMOR; BINDING; AGENTS; IL-10; GAMMA; OXIDE;

D O I：

10.1016/j.micpath.2024.107007

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The present study aimed to evaluate the anti-staphylococcal, antibiofilm, cytotoxicity and trypanocidal activity, mechanisms of parasite death and immunomodulatory effect of CrataBL encapsulated into liposomes (CrataBL-Lipo). CrataBL-Lipo were prepared by the freeze-thaw technique and characterized. Anti-staphylococcal and antibiofilm activities of CrataBL and CrataBL-Lipo were evaluated against standard and clinical strains of Staphylococcus aureus susceptible and resistant. Thus, broth microdilution method was performed to determine the Minimum Inhibitory Concentration (MIC). Antibiofilm activity at subinhibitory concentrations was evaluated using the crystal violet staining method. Cytotoxicity of CrataBL-Lipo was verified in L929 fibroblasts and J774A.1 macrophages by determining the inhibitory concentration necessary to kill 50 % of cells (IC50). Trypanocidal activities of CrataBL-Lipo was evaluated in Trypanosoma cruzi and the efficacy was expressed as the concentration necessary to kill 50 % of parasites (EC50). The mechanisms of parasite death and immunomodulatory effect of CrataBL-Lipo were evaluated using flow cytometry analysis. CrataBL-Lipo presented & Oslash; of 101.9 +/- 1.3 nm (PDI = 0.245), zeta of +33.8 +/- 1.3 mV and %EE = 80 +/- 0.84 %. CrataBL-Lipo presented anti-staphylococcal activity (MIC = 0.56 mg/mL to 0.72 mg/mL). CrataBL-Lipo inhibited 45.4 %-75.6 % of biofilm formation. No cytotoxicity of CrataBL-Lipo was found (IC50 > 100 mg/L). CrataBL-Lipo presented EC50 of 1.1 mg/L, presenting autophagy, apoptosis and necrosis as death profile. In addition, CrataBL-Lipo reduced the production of IL-10 and TNF-alpha levels, causing an immunomodulatory effect. CrataBL-Lipo has a therapeutic potential for the treatment of staphylococcal infections and Chagas disease exhibiting a high degree of selectivity for the microorganism, and immunomodulatory properties.

引用

页数：11

共 76 条

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