Copper(II) complex with 1-allylimidazole induces G2/M cell cycle arrest and suppresses A549 cancer cell growth by attenuating Wnt, JAK-STAT, and TGF-β signaling pathways

被引：0
作者：
Galczynska, Katarzyna [1 ]
Wegierek-Ciuk, Aneta [1 ]
Durlik-Popinska, Katarzyna [1 ]
Zarnowiec, Paulina [1 ]
Kurdziel, Krystyna [2 ]
Arabski, Michal [1 ]
机构：
[1] Jan Kochanowski Univ, Inst Biol, Uniwersytecka 7, PL-25406 Kielce, Poland
[2] Jan Kochanowski Univ, Inst Chem, Uniwersytecka 7, PL-25406 Kielce, Poland
来源：
JOURNAL OF INORGANIC BIOCHEMISTRY | 2025年 / 264卷
关键词：
Copper(II) complex; Anti-tumor activity; Apoptosis; ROS production; DNA damage; WNT pathway inhibition; IN-VITRO; DNA-BINDING; MIXED CHELATE; SCHIFF-BASE; X-RAY; APOPTOSIS; CU(II); PROLIFERATION; CYTOTOXICITY; GENOTOXICITY;
D O I：
10.1016/j.jinorgbio.2024.112791
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The main aim of the study was to investigate the molecular mechanism of action of the potentially anti-cancer agent copper(II) complex with 1-allylimidazole [Cu(1-allim)4(NO3)2] using the A549 lung cancer line, toward which it is selectively cytotoxic. Gene expression analysis showed that the complex caused apoptosis through WNT, JAK-STAT, and TGF-beta pathways. The complex induced DNA damage, ROS production, and depolarization of the mitochondrial membrane, suggesting that its toxicity is likely due to induction of the intrinsic apoptosis pathway. It also arrested the cell cycle at G2/M phase. Particularly noteworthy is that it inhibited the WNT pathway, a target for lung cancer therapies. Its complex mechanism of action may hinder the acquisition of immunity by cancer cells.
引用
收藏
页数：9
相关论文
共 50 条
  • [31] Swertia chirayita suppresses the growth of non-small cell lung cancer A549 cells and concomitantly induces apoptosis via downregulation of JAK1/STAT3 pathway
    Ahmad, Afza
    Tiwari, Rohit Kumar
    Almeleebia, Tahani M.
    Al Fayi, Majed Saad
    Alshahrani, Mohammad Y.
    Ahmad, Irfan
    Abohassan, Mohammad S.
    Saeed, Mohd
    Ansari, Irfan Ahmad
    SAUDI JOURNAL OF BIOLOGICAL SCIENCES, 2021, 28 (11) : 6279 - 6288
  • [32] Induction of G2/M cell cycle arrest and apoptosis by a benz[f]indole-4,9-dione analog in cultured human lung (A549) cancer cells
    Lee, EJ
    Lee, HJ
    Park, HJ
    Min, HY
    Suh, ME
    Chung, HJ
    Lee, SK
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2004, 14 (20) : 5175 - 5178
  • [33] A methoxyflavanone derivative from the Asian medicinal herb (Perilla frutescens) induces p53-mediated G2/M cell cycle arrest and apoptosis in A549 human lung adenocarcinoma
    Amer Ali Abd El-Hafeez
    Takashi Fujimura
    Rikiya Kamei
    Noriko Hirakawa
    Kenji Baba
    Kazuhisa Ono
    Seiji Kawamoto
    Cytotechnology, 2018, 70 : 899 - 912
  • [34] γ-Sitosterol from Acacia nilotica L. induces G2/M cell cycle arrest and apoptosis through c-Myc suppression in MCF-7 and A549 cells
    Sundarraj, Shenbagamoorthy
    Thangam, Ramar
    Sreevani, Vellingiri
    Kaveri, Krishnasamy
    Gunasekaran, Palani
    Achiraman, Shanmugam
    Kannan, Soundarapandian
    JOURNAL OF ETHNOPHARMACOLOGY, 2012, 141 (03) : 803 - 809
  • [35] Molecular mechanisms of luteolin-7-O-glucoside-induced growth inhibition on human liver cancer cells: G2/M cell cycle arrest and caspase-independent apoptotic signaling pathways
    Hwang, Yu-Jin
    Lee, Eun-Ju
    Kim, Haeng-Ran
    Hwang, Kyung-A
    BMB REPORTS, 2013, 46 (12) : 611 - 616
  • [36] Oridonin induces G2/M cell cycle arrest and apoptosis via the PI3K/Akt signaling pathway in hormone-independent prostate cancer cells
    Lu, Jianlei
    Chen, Xiang
    Qu, Shuang
    Yao, Bing
    Xu, Yuexin
    Wu, Jiahui
    Jin, Yucui
    Ma, Changyan
    ONCOLOGY LETTERS, 2017, 13 (04) : 2838 - 2846
  • [37] Crassolide Induces G2/M Cell Cycle Arrest, Apoptosis, and Autophagy in Human Lung Cancer Cells via ROS-Mediated ER Stress Pathways
    Lai, Kuan-Ming
    Wang, Jou-Hsuan
    Lin, Shih-Chao
    Wen, Ya
    Wu, Chao-Liang
    Su, Jui-Hsin
    Chen, Chien-Chin
    Lin, Chi-Chien
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (10)
  • [38] Isopentyl-Deoxynboquinone Induces Mitochondrial Dysfunction and G2/M Phase Cell Cycle Arrest to Selectively Kill NQO1-Positive Pancreatic Cancer Cells
    Jiang, Lingxiang
    Liu, Yingchun
    Tumbath, Soumya
    Boudreau, Matthew W.
    Chatkewitz, Lindsay E.
    Wang, Jiangwei
    Su, Xiaolin
    Zahid, Kashif Rafiq
    Li, Katherine
    Chen, Yaomin
    Yang, Kai
    Hergenrother, Paul J.
    Huang, Xiumei
    ANTIOXIDANTS & REDOX SIGNALING, 2024, 41 (1-3) : 74 - 92
  • [39] Bavachinin Induces G2/M Cell Cycle Arrest and Apoptosis via the ATM/ATR Signaling Pathway in Human Small Cell Lung Cancer and Shows an Antitumor Effect in the Xenograft Model
    Hung, Shih-Ya
    Lin, Shih-Chao
    Wang, Shuzhen
    Chang, Tzu-Jung
    Tung, Yu-Tang
    Lin, Chi-Chien
    Ho, Chi-Tang
    Li, Shiming
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2021, 69 (22) : 6260 - 6270
  • [40] Norwogonin flavone suppresses the growth of human colon cancer cells via mitochondrial mediated apoptosis, autophagy induction and triggering G2/M phase cell cycle arrest
    Wang, Zhang
    Zhang, Qinqin
    Zhou, Ling
    Liu, Gao
    Wu, Quanfeng
    Chen, Changwang
    JOURNAL OF BUON, 2020, 25 (03): : 1449 - 1454
12345