A Novel Bifidobacterium longum Subsp. longum T1 Strain from Cow's Milk: Homeostatic and Antibacterial Activity against ESBL-Producing Escherichia coli

被引：0

作者：

Machulin, Andrey V. ^{[1
,2
]}

Abramov, Vyacheslav M. ^{[1
,3
]}

Kosarev, Igor V. ^{[1
,3
]}

Deryusheva, Evgenia I. ^{[4
]}

Priputnevich, Tatiana V. ^{[3
]}

Panin, Alexander N. ^{[1
]}

Manoyan, Ashot M. ^{[1
]}

Chikileva, Irina O. ^{[5
]}

Abashina, Tatiana N. ^{[2
]}

Blumenkrants, Dmitriy A. ^{[1
]}

Ivanova, Olga E. ^{[1
]}

Papazyan, Tigran T. ^{[6
]}

Nikonov, Ilia N. ^{[7
]}

Suzina, Nataliya E. ^{[2
]}

Melnikov, Vyacheslav G. ^{[8
]}

Khlebnikov, Valentin S. ^{[9
]}

Sakulin, Vadim K. ^{[9
]}

Samoilenko, Vladimir A. ^{[2
]}

Gordeev, Alexey B. ^{[3
]}

Sukhikh, Gennady T. ^{[3
]}

Uversky, Vladimir N. ^{[10
]}

Karlyshev, Andrey V. ^{[11
]}

机构：

[1] Russian State Ctr Anim Feed & Drug Standardizat &, Rosselkhoznadzor Fed State Budgetary Inst, Fed Serv Vet & Phytosanitary Surveillance, Moscow 123022, Russia

[2] Russian Acad Sci, Skryabin Inst Biochem & Physiol Microorganisms, Pushchino Sci Ctr Biol Res, Fed Res Ctr, Pushchino 142290, Russia

[3] Kulakov Natl Med Res Ctr Obstet Gynecol Perinatol, Moscow 117997, Russia

[4] Russian Acad Sci, Inst Biol Instrumentat, Fed Res Ctr, Pushchino Sci Ctr Biol Res, Pushchino 142290, Russia

[5] Minist Hlth Russian Federat, Blokhin Natl Res Ctr Oncol, Moscow 115478, Russia

[6] Alltech Co, Moscow 105062, Russia

[7] St Petersburg State Univ Vet Med, Fed State Budgetary Educ Inst Higher Educ, St Petersburg, Russia

[8] Gabrichevsky Res Inst Epidemiol & Microbiol, Moscow, Russia

[9] Inst Immunol Engn, Lyubuchany 142380, Russia

[10] Univ S Florida, Morsani Coll Med, Dept Mol Med, Tampa, FL 33612 USA

[11] Kingston Univ London, Fac Hlth, Sch Life Sci, Dept Biomol Sci, Kingston Upon Thames KT1 2EF, England

来源：

ANTIBIOTICS-BASEL | 2024年 / 13卷 / 10期

关键词：

Bifidobacterium longum subsp. longum; antagonistic activity; ESBL-producing E. coli; homeostasis; SPECTRUM-BETA-LACTAMASE; INTESTINAL ALKALINE-PHOSPHATASE; BLOOD-STREAM INFECTIONS; KLEBSIELLA-PNEUMONIAE; CTX-M; PATTERN-RECOGNITION; GUT MICROBIOTA; UP-REGULATION; IN-VITRO; ENTEROPATHOGENIC INFECTION;

D O I：

10.3390/antibiotics13100924

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background/Objectives: The global emergence of antibiotic-resistant zooanthroponotic Escherichia coli strains, producing extended-spectrum beta-lactamases (ESBL-E) and persisting in the intestines of farm animals, has now led to the development of a pandemic of extra-intestinal infectious diseases in humans. The search for innovative probiotic microorganisms that eliminate ESBL-E from the intestines of humans and animals is relevant. Previously, we received three isolates of bifidobacteria: from milk of a calved cow (BLLT1), feces of a newborn calf (BLLT2) and feces of a three-year-old child who received fresh milk from this calved cow (BLLT3). Our goal was to evaluate the genetic identity of BLLT1, BLLT2, BLLT3 isolates using genomic DNA fingerprinting (GDF), to study the tolerance, adhesion, homeostatic and antibacterial activity of BLLT1 against ESBL-E. Methods: We used a complex of microbiological, molecular biological, and immunological methods, including next generation sequencing (NGS). Results: GDF showed that DNA fragments of BLLT2 and BLLT3 isolates were identical in number and size to DNA fragments of BLLT1. These data show for the first time the possibility of natural horizontal transmission of BLLT1 through with the milk of a calved cow into the intestines of a calf and the intestines of a child. BLLT1 was resistant to gastric and intestinal stresses and exhibited high adhesive activity to calf, pig, chicken, and human enterocytes. This indicates the unique ability of BLLT1 to inhabit the intestines of animals and humans. We are the first to show that BLLT1 has antibacterial activity against ESBL-E strains that persist in humans and animals. BLLT1 produced 145 +/- 8 mM of acetic acid, which reduced the pH of the nutrient medium from 6.8 to 5.2. This had an antibacterial effect on ESBL-E. The genome of BLLT1 contains ABC-type carbohydrate transporter gene clusters responsible for the synthesis of acetic acid with its antibacterial activity against ESBL-E. BLLT1 inhibited TLR4 mRNA expression induced by ESBL-E in HT-29 enterocytes, and protected the enterocyte monolayers used in this study as a bio-model of the intestinal barrier. BLLT1 increased intestinal alkaline phosphatase (IAP) as one of the main molecular factors providing intestinal homeostasis. Conclusions: BLLT1 shows promise for the creation of innovative functional nutritional products for humans and feed additives for farm animals that will reduce the spread of ESBL-E strains in the food chain.

引用

页数：28

共 170 条

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