Protocol for mapping differential protein-protein interaction networks using affinity purification-mass spectrometry

被引：0
|
作者：
Kaushal, Prashant [1 ,2 ,3 ]
Ummadi, Manisha R. [4 ,5 ,6 ,7 ]
Jang, Gwendolyn M. [4 ,5 ,6 ,7 ]
Delgado, Yennifer [1 ,2 ,3 ]
Makanani, Sara K. [1 ,2 ,3 ]
Alba, Kareem [1 ,2 ,3 ]
Winters, Declan M. [1 ,2 ,3 ]
Blanc, Sophie F. [1 ,2 ,3 ]
Xu, Jiewei [4 ,5 ,6 ,7 ]
Polacco, Benjamin [4 ,5 ,6 ,7 ]
Zhou, Yuan [4 ,5 ,6 ,7 ]
Stevenson, Erica [4 ,5 ,6 ,7 ]
Eckhardt, Manon [4 ,5 ,6 ,7 ]
Zuliani-Alvarez, Lorena [4 ,5 ,6 ,7 ]
Kaake, Robyn [4 ,5 ,6 ,7 ]
Swaney, Danielle L. [4 ,5 ,6 ,7 ]
Krogan, Nevan J. [4 ,5 ,6 ,7 ,8 ]
Bouhaddou, Mehdi [1 ,2 ,3 ]
机构：
[1] Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Inst Quantitat & Computat Biosci, Los Angeles, CA 90095 USA
[3] Univ Calif Los Angeles, Mol Biol Inst, Los Angeles, CA 90095 USA
[4] Univ Calif San Francisco, Quantitat Biosci Inst QBI, San Francisco, CA 94115 USA
[5] Univ Calif San Francisco, QBI Coronavirus Res Grp QCRG, San Francisco, CA 94115 USA
[6] Univ Calif San Francisco, Dept Cellular & Mol Pharmacol, San Francisco 94115, CA USA
[7] Gladstone Inst Data Sci & Biotechnol, J David Gladstone Inst, San Francisco, CA 94158 USA
[8] Univ Calif San Francisco, Dept Bioengn & Therapeut Sci, San Francisco, CA 94115 USA
来源：
STAR PROTOCOLS | 2024年 / 5卷 / 04期
基金：
美国国家卫生研究院;
关键词：
IDENTIFICATION;
D O I：
10.1016/j.xpro.2024.103286
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Proteins congregate into complexes to perform diverse cellular functions. Protein complexes are remodeled by protein-coding mutations or cellular signaling changes, driving phenotypic outcomes in health and disease. We present an affinity purification-mass spectrometry (AP-MS) proteomics protocol to express affinity-tagged "bait"proteins in mammalian cells, identify and quantify purified protein interactors, and visualize differential protein-protein interaction networks between pairwise conditions. Our protocol possesses general applicability to various cell types and biological areas. For complete details on the use and execution of this protocol, please refer to Bouhaddou et al.1
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页数：27
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