Genome-Wide Characterization of Solanum tuberosum CCoAOMT Gene Family and Identification of StCCoAOMT Genes Involved in Anthocyanin Biosynthesis

被引:2
作者
Peng, Yaxuan [1 ]
Sheng, Suao [1 ]
Wang, Tongtong [1 ]
Song, Jiafeng [1 ]
Wang, Daijuan [1 ]
Zhang, Yixuan [1 ]
Cheng, Jielan [1 ]
Zheng, Tingting [1 ]
Lv, Zhaoyan [1 ]
Zhu, Xiaobiao [1 ]
Hou, Hualan [1 ]
机构
[1] Anhui Agr Univ, Sch Hort, Hefei 230036, Peoples R China
基金
中国国家自然科学基金;
关键词
caffeoyl-CoA-O methyltransferase; expression pattern; functional analysis; StCCoAOMT10; subcellular localization; COENZYME-A; 3-O-METHYLTRANSFERASE; ADENOSYL-L-METHIONINE; O-METHYLTRANSFERASE; TRANSCRIPTION FACTORS; LIGNIN BIOSYNTHESIS; METHYLATION; FLAVONOIDS; ACCUMULATION; EXPRESSION; EVOLUTION;
D O I
10.3390/genes15111466
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Background: The caffeoyl-CoA-O methyltransferase (CCoAOMT) family plays essential roles in the methylation of various secondary metabolites, including anthocyanins. Despite the wide identification of the CCoAOMT family in plants, the characterization and function of CCoAOMT protein members in Solanum tuberosum remain poorly understood. Methods and Results: In this study, a total of 12 StCCoAOMT members were identified in the genome of S. tuberosum using the Blastp and HMM search and were unevenly located on eight chromosomes. Collinearity analysis revealed that four tandem duplicated gene pairs and two segmental duplicated gene pairs existed in the S. tuberosum genome, demonstrating that duplication events play a key role in the expansion of the CCoAOMT family. All StCCoAOMTs were clustered into group I and group II based on phylogenetic analysis, which was further verified by the conserved motifs and gene structures analysis. The cis-acting elements analysis illustrated that StCCoAOMTs might be important for photosynthesis, hormone responses, and abiotic stress. Expression analysis demonstrated that StCCoAOMT genes have diverse transcript levels in various tissues and that StCCoAOMT10 was significantly expressed in purple potatoes with abundant anthocyanin content according to RNA-seq data and qRT-PCR assays. In addition, the subcellular localization assay validated that the StCCoAOMT10 protein was mainly localized in the cytoplasm and nucleus. Conclusions: These results will be of great importance for a better understanding of the features of CCoAOMT family members, especially of the candidate genes involved in the methylation of anthocyanins in S. tuberosum, and also for improving the nutritional quality of S. tuberosum.
引用
收藏
页数:17
相关论文
共 75 条
[1]   Four specific isogenes of the anthocyanin metabolic pathway are systematically co-expressed with the red colour of grape berries [J].
Ageorges, A ;
Fernandez, L ;
Vialet, S ;
Merdinoglu, D ;
Terrier, N ;
Romieu, C .
PLANT SCIENCE, 2006, 170 (02) :372-383
[2]   MYB diplotypes at the color locus affect the ratios of tri/di-hydroxylated and methylated/non-methylated anthocyanins in grape berry skin [J].
Azuma, Akifumi ;
Ban, Yusuke ;
Sato, Akihiko ;
Kono, Atsushi ;
Shiraishi, Mikio ;
Yakushiji, Hiroshi ;
Kobayashi, Shozo .
TREE GENETICS & GENOMES, 2015, 11 (02)
[3]   MEME SUITE: tools for motif discovery and searching [J].
Bailey, Timothy L. ;
Boden, Mikael ;
Buske, Fabian A. ;
Frith, Martin ;
Grant, Charles E. ;
Clementi, Luca ;
Ren, Jingyuan ;
Li, Wilfred W. ;
Noble, William S. .
NUCLEIC ACIDS RESEARCH, 2009, 37 :W202-W208
[4]   A Set of Regioselective O-Methyltransferases Gives Rise to the Complex Pattern of Methoxylated Flavones in Sweet Basil [J].
Berim, Anna ;
Hyatt, David C. ;
Gang, David R. .
PLANT PHYSIOLOGY, 2012, 160 (02) :1052-1069
[5]   Crops that feed the world 8: Potato: are the trends of increased global production sustainable? [J].
Birch, Paul R. J. ;
Bryan, Glenn ;
Fenton, Brian ;
Gilroy, Eleanor M. ;
Hein, Ingo ;
Jones, John T. ;
Prashar, Ankush ;
Taylor, Mark A. ;
Torrance, Lesley ;
Toth, Ian K. .
FOOD SECURITY, 2012, 4 (04) :477-508
[6]   Quercetin Protects Primary Human Osteoblasts Exposed to Cigarette Smoke through Activation of the Antioxidative Enzymes HO-1 and SOD-1 [J].
Braun, Karl F. ;
Ehnert, Sabrina ;
Freude, Thomas ;
Egana, Jose T. ;
Schenck, Thilo L. ;
Buchholz, Arne ;
Schmitt, Andreas ;
Siebenlist, Sebastian ;
Schyschka, Lilianna ;
Neumaier, Markus ;
Stoeckle, Ulrich ;
Nussler, Andreas K. .
THESCIENTIFICWORLDJOURNAL, 2011, 11 :2348-2357
[7]   Modulation of Phytohormone Signaling: A Primary Function of Flavonoids in Plant-Environment Interactions [J].
Brunetti, Cecilia ;
Fini, Alessio ;
Sebastiani, Federico ;
Gori, Antonella ;
Tattini, Massimiliano .
FRONTIERS IN PLANT SCIENCE, 2018, 9
[8]   A flavonol O-methyltransferase from Catharanthus roseus performing two sequential methylations [J].
Cacace, S ;
Schröder, G ;
Wehinger, E ;
Strack, D ;
Schmidt, J ;
Schröder, J .
PHYTOCHEMISTRY, 2003, 62 (02) :127-137
[9]   TBtools-II: A "one for all, all for one"bioinformatics platform for biological big-data mining [J].
Chen, Chengjie ;
Wu, Ya ;
Li, Jiawei ;
Wang, Xiao ;
Zeng, Zaohai ;
Xu, Jing ;
Liu, Yuanlong ;
Feng, Junting ;
Chen, Hao ;
He, Yehua ;
Xia, Rui .
MOLECULAR PLANT, 2023, 16 (11) :1733-1742
[10]   Cell-specific and conditional expression of caffeoyl-coenzyme A-3-O-methyltransferase in poplar [J].
Chen, CY ;
Meyermans, H ;
Burggraeve, B ;
De Rycke, RM ;
Inoue, K ;
De Vleesschauwer, V ;
Steenackers, M ;
Van Montagu, MC ;
Engler, GJ ;
Boerjan, WA .
PLANT PHYSIOLOGY, 2000, 123 (03) :853-867