Development of hydro-distillation extraction and macroporous resin enrichment method for GC-MS chemical profiling of volatile components in the dried root of Gentiana dahurica Fisch

被引：0

作者：

Ji, Wen ^{[1
]}

Nie, Xinkai ^{[1
]}

Sun, Lang ^{[2
]}

Wang, Yaxuan ^{[1
]}

Tian, Sheng ^{[1
,3
]}

Li, Duxin ^{[1
]}

机构：

[1] Soochow Univ, Coll Pharmaceut Sci, Suzhou, Peoples R China

[2] Anlab Jiangsu Pharmaceut Technol Co Ltd, Suzhou, Peoples R China

[3] Soochow Univ, Jiangsu Prov Engn Res Ctr Precis Diagnost & Therap, Suzhou, Peoples R China

来源：

NATURAL PRODUCT RESEARCH | 2024年

关键词：

<italic>Gentiana dahurica</italic> Fisch; volatile component; GC-MS; extraction methods; antimicrobial activity;

D O I：

10.1080/14786419.2024.2418448

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The dried root of Gentiana dahurica Fisch. has an intensive flavour, which suggests the presence of volatile compounds. Thus, this study aimed to develop an effective extraction method for GC-MS profiling of volatile compounds of G. dahurica. Hydro-distillation and subsequent resin enrichment (HDRE) was developed and applied to the analysis of G. dahurica in comparison with simultaneous distillation and extraction (SDE), and ultrasonic assisted dichloromethane extraction (uDCM). The major components were azulene (33.83%), 1-hexanoic acid (14.19%) and octanoic acid (11.39%) in the HDRE; octanoic acid (17.24%), nonanoic acid (13.29%) and 2,4-di-t-butylphenol (12.73%) in the SDE; and alpha-amyrin (25.05%), beta-amyrin acetate (15.11%) and beta-amyrone (4.61%) in the uDCM. Moreover, the HDRE extract was shown to have certain antimicrobial activity against four bacterial strains. Thus, the HDRE method is a promising sample preparation method for GC-MS chemical profiling of volatile components of the dried roots of medicinal plants.

引用

页数：7