The diagnostic value of metagenomic next-generation sequencing in suspected pulmonary tuberculosis patients with scarce sputum or negative sputum etiological test results

被引：0
|
作者：
Xiong, Wanfeng [1 ]
Dong, Liang [1 ]
Zhu, Ning [1 ]
Zhou, Daibing [1 ]
Li, Shuanghui [1 ]
Lv, Junzhu [1 ]
Xu, Mengqi [1 ]
Zhang, Yuhai [2 ]
Li, Shengqing [1 ]
机构：
[1] Fudan Univ, Huashan Hosp, Dept Pulm & Crit Care Med, 12 Urumqi Middle Rd, Shanghai, Peoples R China
[2] Air Force Med Univ, Dept Hlth Stat, Xian, Peoples R China
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2025年 / 111卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Diagnostic value; Metagenomic next-generation sequencing (mNGS); Mycobacterium tuberculosis complex (MTBC); Pulmonary tuberculosis (PTB); Receiver operating curve (ROC); TRANSMISSION;
D O I：
10.1016/j.diagmicrobio.2024.116633
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Objective: To compare the diagnostic value between mNGS and conventional tests in suspected pulmonary tuberculosis (PTB) patients with scarce sputum or with negative sputum etiological test results. Methods: We enrolled eligible patients admitted to our department from 2018 to 2021. Their bronchoalveolar lavage fluid (BALF) and lung biopsy tissue samples were sent for mNGS and conventional tests. The diagnostic value of mNGS was compared respectively with that of conventional tests. Results: 94 of 226 enrolled patients were diagnosed PTB. The diagnostic concordance rate of mNGS was significantly higher than that of acid-fast staining in all samples (p < 0.001), as well as that of culture in BALF samples (p = 0.011). mNGS in parallel with conventional tests had significantly higher AUC than mNGS alone (p = 0.013). Conclusions: mNGS has higher diagnostic value than some conventional tests. mNGS in parallel with conventional tests is more reliable to accurately diagnose these suspected PTB patients.
引用
收藏
页数：6
相关论文
共 50 条
  • [1] Diagnostic Accuracy of Metagenomic Next-Generation Sequencing in Sputum-Scarce or Smear-Negative Cases with Suspected Pulmonary Tuberculosis
    Zhu, Ning
    Zhou, Daibing
    Li, Shengqing
    BIOMED RESEARCH INTERNATIONAL, 2021, 2021
  • [2] The etiological diagnostic value of metagenomic next-generation sequencing in suspected community-acquired pneumonia
    Liu, Mengling
    Zhang, Haiyue
    Li, Liangyu
    Mao, Jieyu
    Li, Ruiyun
    Yin, Jing
    Wu, Xiaojun
    BMC INFECTIOUS DISEASES, 2024, 24 (01)
  • [3] Comparison of 3 diagnostic methods for pulmonary tuberculosis in suspected patients with negative sputum smear or no sputum
    Cheng, Xiaopeng
    Chen, Lerong
    Wan, Wenli
    Peng, Jianping
    Wu, Liangliang
    Xin, Jing
    Cai, Jianying
    MEDICINE, 2024, 103 (06) : E37039
  • [4] Diagnostic Significance of Metagenomic Next-Generation Sequencing in Immunocompromised Patients With Suspected Pulmonary Infection
    Zheng, Xi
    Zou, Wei
    Zou, Shumei
    Ye, Jia
    Bao, Zhenming
    Song, Yingfang
    IMMUNOLOGY, 2025,
  • [5] Original Diagnostic value of bronchoalveolar lavage and bronchial washing in sputum -scarce or smear -negative cases with suspected pulmonary tuberculosis: a randomized study
    Kim, Y. W.
    Kwon, B. S.
    Lim, S. Y.
    Lee, Y. J.
    Cho, Y-J
    Yoon, H., I
    Lee, J. H.
    Lee, C-T
    Park, J. S.
    CLINICAL MICROBIOLOGY AND INFECTION, 2020, 26 (07) : 911 - 916
  • [6] The Application of Metagenomic Next-Generation Sequencing in Detection of Pathogen in Bronchoalveolar Lavage Fluid and Sputum Samples of Patients with Pulmonary Infection
    Shi, Wanghui
    Zhu, Shanshan
    COMPUTATIONAL AND MATHEMATICAL METHODS IN MEDICINE, 2021, 2021
  • [7] Diagnostic Value of Metagenomic Next-Generation Sequencing for Pneumonia in Immunocompromised Patients
    Li, Jun
    Zhou, Chao-E.
    Wei, Shan-Chen
    Wang, Li-Na
    Shi, Ming-Wei
    Sun, Chun-Ping
    Lin, Lian-Jun
    Liu, Xin-Min
    CANADIAN JOURNAL OF INFECTIOUS DISEASES & MEDICAL MICROBIOLOGY, 2022, 2022
  • [8] BALF metagenomic next-generation sequencing analysis in hematological malignancy patients with suspected pulmonary infection: clinical significance of negative results
    Deng, Zuqun
    Tang, Yishu
    Tu, Yixuan
    Liu, Mei
    Cheng, Qian
    Zhang, Jian
    Liu, Feiyang
    Li, Xin
    FRONTIERS IN MEDICINE, 2023, 10
  • [9] The diagnostic value of metagenomic next-generation sequencing in infectious diseases
    Duan, Hongxia
    Li, Xuan
    Mei, Aihong
    Li, Ping
    Liu, Yang
    Li, Xiaofeng
    Li, Weiwei
    Wang, Changhui
    Xie, Shuanshuan
    BMC INFECTIOUS DISEASES, 2021, 21 (01)
  • [10] Sputum induction to improve the diagnostic yield in patients with suspected pulmonary tuberculosis
    Li, LM
    Bai, LQ
    Yang, HL
    Xiao, CF
    Tang, RY
    Chen, YF
    Chen, SM
    Liu, SS
    Zhang, SN
    Ou, YH
    Niu, TIM
    INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE, 1999, 3 (12) : 1137 - 1139
12345