Improving survival and growth of caprine preantral follicles cultured in medium commonly used for MSC: Role of oxidative stress regulation and epigenetic changes

被引：0

作者：

Silva, Ana F. B. ^{[1
]}

Lima, Laritza F. ^{[1
]}

Ferreira, Anna C. A. ^{[1
]}

Oliveira, Ariclecio C. ^{[2
]}

Neto, Napolea M. A. ^{[3
]}

Alves, Benner G. ^{[4
]}

Rodrigues, Ana P. R. ^{[1
]}

Gastal, Eduardo L. ^{[5
]}

Bordignon, Vilceu ^{[6
]}

Figueiredo, Jose R. ^{[1
]}

机构：

[1] Univ Estadual Ceara, Lab Manipulat Oocytes & Preantral Follicles LAMOFO, Fortaleza, CE, Brazil

[2] Univ Estadual Ceara, Super Inst Biomed Sci, Fortaleza, CE, Brazil

[3] Univ Fed Piaui, Integrated Nucleus Morphol & Res Stem Cells, Teresina, PI, Brazil

[4] Concept Biosci Inc, Berkeley, CA USA

[5] Southern Illinois Univ, Sch Agr Sci, Anim Sci, Carbondale, IL 62901 USA

[6] McGill Univ, Dept Anim Sci, Ste Anne De Bellevue, PQ, Canada

来源：

DOMESTIC ANIMAL ENDOCRINOLOGY | 2025年 / 90卷

关键词：

In vitro culture; MSC medium; Preantral follicles; ROS; H3K4me3; H3K9me3;

D O I：

10.1016/j.domaniend.2024.106890

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

This study evaluated the efficiency of in vitro culture of preantral follicles (PAF) in a commonly used medium for mesenchymal stem cell (MSC) culture. Parameters assessed included follicle survival, growth, stromal cell density, levels of reduced thiols and reactive oxygen species, epigenetic changes, cell apoptosis, and mRNA abundance. Caprine ovarian tissues were cultured for 1 or 7 days in either PAF or MSC-common media, with uncultured tissues serving as controls. The MSC medium exhibited increased follicular survival and growth and remodeled stromal density potentially through the regulation of oxidative stress and epigenetic changes compared to the PAF medium. In conclusion, our results highlight the importance of the MSC medium in enhancing follicular survival and growth, changing the stromal cell density, as well as in regulating the medium oxidative stress and epigenetic changes during the in vitro culture of caprine PAF.

引用

页数：6

共 3 条

[1] Anti-Mullerian hormone reduces growth rate without altering follicular survival in isolated caprine preantral follicles cultured in vitro
Rocha, R. M. P.
Lima, L. F.
Brito, I. R.
Silva, G. M.
Correia, H. H. V.
Ribeiro de Sa, N. A.
Ferreira, A. C. A.
Sales, A. D.
Lobo, C. H.
Campello, C. C.
Smitz, J.
Wheeler, M. B.
Figueiredo, J. R.
REPRODUCTION FERTILITY AND DEVELOPMENT, 2017, 29 (06) : 1144 - 1154
[2] Dynamic medium containing growth differentiation factor-9 and FSH maintains survival and promotes in vitro growth of caprine preantral follicles after long-term in vitro culture
Alves, A. M. C. V.
Chaves, R. N.
Rocha, R. M. P.
Lima, L. F.
Andrade, P. M.
Lopes, C. A. P.
Souza, C. E. A.
Moura, A. A. A.
Campello, C. C.
Bao, S. N.
Smitz, J.
Figueiredo, J. R.
REPRODUCTION FERTILITY AND DEVELOPMENT, 2013, 25 (06) : 955 - 965
[3] Dynamic Medium Containing Kit Ligand and Follicle-Stimulating Hormone Promotes Follicular Survival, Activation, and Growth during Long-Term in vitro Culture of Caprine Preantral Follicles
Lima, I. M. T.
Celestino, J. J. H.
Faustino, L. R.
Magalhaes-Padilha, D. M.
Rossetto, R.
Brito, I. R.
Donato, M. A. M.
Lopes, C. A. P.
Campello, C. C.
Peixoto, C. A.
Figueiredo, J. R.
Rodrigues, A. P. R.
CELLS TISSUES ORGANS, 2012, 195 (03) : 260 - 271

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